ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2008
False negative PCR despite high levels of JC virus DNA in spinal fluid: Implications for diagnostic testing.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Article
- John Cunningham Virus and Progressive Multifocal Leukoencephalopathy: A Falsely Played Diagnosis.Diseases (Basel, Switzerland) · 2024Review
- Progressive multifocal leukoencephalopathy: a retrospective study of the last 12 years in a tertiary-care hospital.Journal of neurovirology · 2023Review
- Undetectable JC virus CSF PCR in patients with JC virus-induced progressive multifocal leukoencephalopathy.Journal of neurovirology · 2023Review
- Assessment and Diagnosis of HIV-Associated Dementia.Viruses · 2023Review
- Answer to August 2022 Photo Quiz.Journal of clinical microbiology · 2022Article
- Flaccid Brachial Monoplegia As Initial Presentation in a Patient With Progressive Multifocal Leukoencephalopathy.Cureus · 2022Article
- Progressive multifocal leukoencephalopathy successfully treated with mefloquine and literature review.Encephalitis (Seoul, Korea) · 2021Article
- A new approach to identifying pathogens, with particular regard to viruses, based on capillary electrophoresis and other analytical techniques.Trends in analytical chemistry : TRAC · 2021Review
- Four cases of progressive multifocal leukoencephalopathy in iatrogenic immunocompromised patients.eNeurologicalSci · 2020Article
- A comparative molecular characterization of AMDV strains isolated from cases of clinical and subclinical infection.Virus genes · 2018Article
- Progressive multifocal leukoencephalopathy in a patient with lymphoma and presumptive hyper IgE syndrome.Journal of neurovirology · 2017Article
- Brain Biopsy Is More Reliable than the DNA test for JC Virus in Cerebrospinal Fluid for the Diagnosis of Progressive Multifocal Leukoencephalopathy.Internal medicine (Tokyo, Japan) · 2017Article
- JC Virus PCR Detection Is Not Infallible: A Fulminant Case of Progressive Multifocal Leukoencephalopathy with False-Negative Cerebrospinal Fluid Studies despite Progressive Clinical Course and Radiological Findings.Case reports in neurological medicine · 2015Article
- [A rare cause of a progressive speech impairment].Medizinische Klinik, Intensivmedizin und Notfallmedizin · 2014Article
- Laboratory diagnosis of viral infection.Handbook of clinical neurology · 2014Review
- Towards multitarget testing in molecular microbiology.International journal of microbiology · 2013Review
- Progressive multifocal leukoencephalopathy: clinical and molecular aspects.Reviews in medical virology · 2012Review
- Reevaluating and optimizing polyomavirus BK and JC real-time PCR assays to detect rare sequence polymorphisms.Journal of clinical microbiology · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Genome amplification methods such as polymerase chain reaction (PCR) have revolutionized our ability to detect viruses in spinal fluids of patients with neurologic diseases. It is not as well appreciated among clinicians that PCR protocols, quality assurance, and technical expertise vary significantly among laboratories. In a multi-laboratory blinded study of herpes simplex virus PCR, the most widely used and best validated CSF PCR assay, low-level positives were often missed and false positives were not uncommon [Schloss L, van Loon AM, Cinque P, Cleator G, Echevarria JM, Falk KI, et al. An international external quality assessment of nucleic acid amplification of herpes simplex virus. J Clin Virol 2003;28(2):175-85]. In addition, genome variability and mutations, which are increasingly recognized for a number of different viruses, can lead to falsely low or negative results. Both clinicians and laboratories must recognize the limitations of PCR, since misleading results may have serious consequences. We present here a case of a rapidly progressive, fatal neurologic illness in a young mother, whose CSF JCV DNA PCR at a reference laboratory was falsely negative. Ultimately, brain biopsy established the diagnosis of progressive multifocal leukoencephalopathy (PML). Repeat PCR testing of the same CSF targeting a different region of the genome yielded a high positive result.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.