ArticleAnalytical chemistry2008
Combining microdialysis, NanoLC-MS, and MALDI-TOF/TOF to detect neuropeptides secreted in the crab, Cancer borealis.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Neuropeptide Diversity Encoded in Newly Sequenced Crustacean Genomes Reveals Signaling Roles during Feeding.ACS chemical neuroscience · 2026Article
- Decoding Neuropeptide Complexity: Advancing Neurobiological Insights from Invertebrates to Vertebrates through Evolutionary Perspectives.ACS chemical neuroscience · 2025Review
- An Updated Guide to the Identification, Quantitation, and Imaging of the Crustacean Neuropeptidome.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Application of microdialysis combined with UHPLC-QTOF/MS to screen for endogenous metabolites in aquatic organisms as biomarkers of exposure to an emerging contaminant, triclosan.Analytical and bioanalytical chemistry · 2023Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Mass Spectrometry Quantification, Localization, and Discovery of Feeding-Related Neuropeptides inACS chemical neuroscience · 2021Article
- Multifaceted Mass Spectrometric Investigation of Neuropeptide Changes in Atlantic Blue Crab, Callinectes sapidus, in Response to Low pH Stress.Journal of proteome research · 2019Article
- Relative Quantitation of Neuropeptides at Multiple Developmental Stages of the American Lobster Using N, N-Dimethyl Leucine Isobaric Tandem Mass Tags.ACS chemical neuroscience · 2018Article
- Microdialysis Coupled with LC-MS/MS for In Vivo Neurochemical Monitoring.The AAPS journal · 2017Article
- Investigation of signaling molecules and metabolites found in crustacean hemolymph via in vivo microdialysis using a multifaceted mass spectrometric platform.Electrophoresis · 2016Article
- In Silico Prediction of Neuropeptides/Peptide Hormone Transcripts in the Cheilostome Bryozoan Bugula neritina.PloS one · 2016Article
- Reducing adsorption to improve recovery and in vivo detection of neuropeptides by microdialysis with LC-MS.Analytical chemistry · 2015Article
- Mass spectrometric analysis of spatio-temporal dynamics of crustacean neuropeptides.Biochimica et biophysica acta · 2015Review
- Mass spectrometric measurement of neuropeptide secretion in the crab, Cancer borealis, by in vivo microdialysis.The Analyst · 2015Article
- Data-independent MS/MS quantification of neuropeptides for determination of putative feeding-related neurohormones in microdialysate.ACS chemical neuroscience · 2015Article
- Article
- Rapid preconcentration for liquid chromatography-mass spectrometry assay of trace level neuropeptides.Journal of the American Society for Mass Spectrometry · 2013Article
- Emerging trends in in vivo neurochemical monitoring by microdialysis.Current opinion in chemical biology · 2013Review
- Function-driven discovery of neuropeptides with mass spectrometry-based tools.Protein and peptide letters · 2013Review
- Mass spectrometric detection of neuropeptides using affinity-enhanced microdialysis with antibody-coated magnetic nanoparticles.Analytical chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microdialysis is a useful technique for sampling neuropeptides in vivo, and decapod crustaceans are important model organisms for studying how these peptides regulate physiological processes. However, to date, no microdialysis procedure has been reported for sampling neuropeptides from crustaceans. Here we report the first application of microdialysis to sample neuropeptides from the hemolymph of the crab, Cancer borealis. Microdialysis probes were implanted into the pericardial region of live crabs, and the resulting dialysates were desalted, concentrated, and analyzed by LC-ESI-QTOF and MALDI-TOF/TOF mass spectrometry. Analysis of in vitro microdialysates of hemolymph revealed more neuropeptides and fewer protein fragments than hemolymph prepared by typical analysis methods. Mass spectra of in vivo dialysates displayed neuropeptides from 10 peptide families, including the RFamide, allatostatin, and orcokinin families. In addition, GAHKNYLRFa, SDRNFLRFa, and TNRNFLRFa were sequenced from hemolymph dialysates. The detection of these neuropeptides in the hemolymph suggests that they are functioning as hormones as well as neuromodulators. In vivo microdialysis offers the capability to further study these and other neuropeptides in crustacean hemolymph, complementing current tissue-based studies and extending our knowledge of hormonal regulation of physiological states.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.