ArticleBMC genomics2008
Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 48 citations in OpenAlex.
- A conserved trypanosomatid differentiation regulator controls substrate attachment and morphological development in Trypanosoma congolense.PLoS pathogens · 2024Article
- Review
- Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast.Current protocols · 2021Article
- African trypanosomes.Parasites & vectors · 2019Article
- The Structure of a Conserved Telomeric Region Associated with Variant Antigen Loci in the Blood Parasite Trypanosoma congolense.Genome biology and evolution · 2018Article
- Transcriptional Regulation of Telomeric Expression Sites and Antigenic Variation in Trypanosomes.Current genomics · 2018Review
- Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology.Chromosoma · 2016Review
- The within-host dynamics of African trypanosome infections.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Genetic recombination between human and animal parasites creates novel strains of human pathogen.PLoS neglected tropical diseases · 2015Article
- Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A short bifunctional element operates to positively or negatively regulate ESAG9 expression in different developmental forms of Trypanosoma brucei.Journal of cell science · 2013Article
- Trypanosoma brucei gambiense adaptation to different mammalian sera is associated with VSG expression site plasticity.PloS one · 2013Article
- A cell-surface phylome for African trypanosomes.PLoS neglected tropical diseases · 2013Article
- The trypanosomatid-specific N terminus of RPA2 is required for RNA polymerase I assembly, localization, and function.Eukaryotic cell · 2012Article
- No recent adaptive selection on the apyrase of Mediterranean Phlebotomus: implications for using salivary peptides to vaccinate against canine leishmaniasis.Evolutionary applications · 2012Article
- Differences between Trypanosoma brucei gambiense groups 1 and 2 in their resistance to killing by trypanolytic factor 1.PLoS neglected tropical diseases · 2011Article
- Cloning of the repertoire of individual Plasmodium falciparum var genes using transformation associated recombination (TAR).PloS one · 2011Article
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- Developmental regulation and extracellular release of a VSG expression-site-associated gene product from Trypanosoma brucei bloodstream forms.Journal of cell science · 2010Article
- The genome sequence of Trypanosoma brucei gambiense, causative agent of chronic human african trypanosomiasis.PLoS neglected tropical diseases · 2010Article
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6 authors at 3 institutions in 1 country.
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Abstract
backgroundAfrican trypanosomes (including Trypanosoma brucei) are unicellular parasites which multiply in the mammalian bloodstream. T. brucei has about twenty telomeric bloodstream form Variant Surface Glycoprotein (VSG) expression sites (BESs), of which one is expressed at a time in a mutually exclusive fashion. BESs are polycistronic transcription units, containing a variety of families of expression site associated genes (ESAGs) in addition to the telomeric VSG. These polymorphic ESAG families are thought to play a role in parasite-host adaptation, and it has been proposed that ESAG diversity might be related to host range. Analysis of the genetic diversity of these telomeric gene families has been confounded by the underrepresentation of telomeric sequences in standard libraries. We have previously developed a method to selectively isolate sets of trypanosome BES containing telomeres using Transformation associated recombination (TAR) cloning in yeast.
resultsHere we describe the isolation of repertoires of BES containing telomeres from three trypanosome subspecies: Trypanosoma brucei gambiense DAL 972 (causative agent of West-African trypanosomiasis), T. b. brucei EATRO 2340 (a nonhuman infective strain) and T. equiperdum STIB 818 (which causes a sexually transmitted disease in equines). We have sequenced and analysed the genetic diversity at four BES loci (BES promoter region, ESAG6, ESAG5 and ESAG2) from these three trypanosome BES repertoires.
conclusionWith the exception of ESAG2, the BES sequence repertoires derived from T. b. gambiense are both less diverse than and nearly reciprocally monophyletic relative to those from T. b. brucei and T. equiperdum. Furthermore, although we find evidence for adaptive evolution in all three ESAG repertoires in T. b. brucei and T. equiperdum, only ESAG2 appears to be under diversifying selection in T. b. gambiense. This low level of variation in the T. b. gambiense BES sequence repertoires is consistent both with the relatively narrow host range of this subspecies and its apparent long-term clonality. However, our data does not show a clear correlation between size of trypanosome host range and either number of BESs or extent of ESAG genetic diversity.
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