ArticleBMC pulmonary medicine2008
Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: lack of interaction with nitric oxide.
Article in BMC pulmonary medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Eosinophils enhance WNT-5a and TGF-β1 genes expression in airway smooth muscle cells and promote their proliferation by increased extracellular matrix proteins production in asthma.BMC pulmonary medicine · 2016Trial
- Loss of Rab44 attenuates ovalbumin-induced allergic airway inflammation by modulating immune responses and eosinophil function.Frontiers in immunology · 2026Article
- Eosinophils as potential biomarkers in respiratory viral infections.Frontiers in immunology · 2023Review
- An open microfluidic coculture model of fibroblasts and eosinophils to investigate mechanisms of airway inflammation.Frontiers in bioengineering and biotechnology · 2022Article
- In utero exposure to genistein decreased intranasal house dust mite-induced respiratory allergy in middle-aged male B6C3F1 offspring.Toxicology letters · 2020Article
- Urine Proteomic Study in OAB Patients-Preliminary Report.Journal of clinical medicine · 2020Article
- Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS.Chinese medicine · 2018Article
- Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis.PLoS neglected tropical diseases · 2017Article
- Pathomechanism of Interstitial Cystitis/Bladder Pain Syndrome and Mapping the Heterogeneity of Disease.International neurourology journal · 2016Review
- CD63 is tightly associated with intracellular, secretory events chaperoning piecemeal degranulation and compound exocytosis in human eosinophils.Journal of leukocyte biology · 2016Article
- In Utero exposure to genistein enhanced intranasal house dust mite allergen-induced respiratory sensitization in young adult B6C3F1 mice.Toxicology letters · 2016Article
- Suppression of Eosinophil Integrins Prevents Remodeling of Airway Smooth Muscle in Asthma.Frontiers in physiology · 2016Article
- SOCS3 silencing attenuates eosinophil functions in asthma patients.International journal of molecular sciences · 2015Article
- Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.Journal of inflammation (London, England) · 2015Article
- CCR3 Blockade Attenuates Eosinophilic Ileitis and Associated Remodeling.The American journal of pathology · 2011Article
- Eosinophil and T cell markers predict functional decline in COPD patients.Respiratory research · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAirway eosinophilia is considered a central event in the pathogenesis of asthma. The toxic components of eosinophils are thought to be important in inducing bronchial mucosal injury and dysfunction. Previous studies have suggested an interaction between nitric oxide (NO) and chemokines in modulating eosinophil functions, but this is still conflicting. In the present study, we have carried out functional assays (adhesion and degranulation) and flow cytometry analysis of adhesion molecules (VLA-4 and Mac-1 expression) to evaluate the interactions between NO and CC-chemokines (eotaxin and RANTES) in human eosinophils.
methodsEosinophils were purified using a percoll gradient followed by immunomagnetic cell separator. Cell adhesion and degranulation were evaluated by measuring eosinophil peroxidase (EPO) activity, whereas expression of Mac-1 and VLA-4 was detected using flow cytometry.
resultsAt 4 h incubation, both eotaxin (100 ng/ml) and RANTES (1000 ng/ml) increased by 133% and 131% eosinophil adhesion, respectively. L-NAME alone (but not D-NAME) also increased the eosinophil adhesion, but the co-incubation of L-NAME with eotaxin or RANTES did not further affect the increased adhesion seen with chemokines alone. In addition, L-NAME alone (but not D-NAME) caused a significant cell degranulation, but it did not affect the CC-chemokine-induced cell degranulation. Incubation of eosinophils with eotaxin or RANTES, in absence or presence of L-NAME, did not affect the expression of VLA-4 and Mac-1 on eosinophil surface. Eotaxin and RANTES (100 ng/ml each) also failed to elevate the cyclic GMP levels above baseline in human eosinophils.
conclusionEotaxin and RANTES increase the eosinophil adhesion to fibronectin-coated plates and promote cell degranulation by NO-independent mechanisms. The failure of CC-chemokines to affect VLA-4 and Mac-1 expression suggests that changes in integrin function (avidity or affinity) are rather involved in the enhanced adhesion.
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