ArticleMolecular pain2008
Proteomic profiling of neuromas reveals alterations in protein composition and local protein synthesis in hyper-excitable nerves.
Article in Molecular pain, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Article
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- Deciphering the molecular landscape of human peripheral nerves: implications for diabetic peripheral neuropathy.bioRxiv : the preprint server for biology · 2024Article
- Neuroma Analysis in Humans: Standardizing Sample Collection and Documentation.The Journal of surgical research · 2024Article
- Traumatic neuromas of peripheral nerves: Diagnosis, management and future perspectives.Frontiers in neurology · 2022Review
- Role of Potassium Ions Quantum Tunneling in the Pathophysiology of Phantom Limb Pain.Brain sciences · 2020Article
- Proteomic profiling of proteins in the dorsal horn of the spinal cord in dairy cows with chronic lameness.PloS one · 2020Article
- Spinal Reactive Oxygen Species and Oxidative Damage Mediate Chronic Pain in Lame Dairy Cows.Animals : an open access journal from MDPI · 2019Article
- Using dynamic clamp to quantify pathological changes in the excitability of primary somatosensory neurons.The Journal of physiology · 2018Article
- Translational Control Mechanisms in Persistent Pain.Trends in neurosciences · 2018Review
- Region-Resolved Quantitative Proteome Profiling Reveals Molecular Dynamics Associated With Chronic Pain in the PNS and Spinal Cord.Frontiers in molecular neuroscience · 2018Article
- The Proteomics of Intrathecal Analgesic agents for Chronic Pain.Current neuropharmacology · 2017Review
- Standardized Profiling of The Membrane-Enriched Proteome of Mouse Dorsal Root Ganglia (DRG) Provides Novel Insights Into Chronic Pain.Molecular & cellular proteomics : MCP · 2016Article
- Resveratrol suppresses glial activation and alleviates trigeminal neuralgia via activation of AMPK.Journal of neuroinflammation · 2016Article
- Exploring novel paths towards protein signatures of chronic pain.Molecular pain · 2016Article
- Article
- Axonal protein synthesis and the regulation of primary afferent function.Developmental neurobiology · 2014Review
- An approach to identify microRNAs involved in neuropathic pain following a peripheral nerve injury.Frontiers in neuroscience · 2014Article
- Proteomic and functional annotation analysis of injured peripheral nerves reveals ApoE as a protein upregulated by injury that is modulated by metformin treatment.Molecular pain · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Neuropathic pain may arise following peripheral nerve injury though the molecular mechanisms associated with this are unclear. We used proteomic profiling to examine changes in protein expression associated with the formation of hyper-excitable neuromas derived from rodent saphenous nerves. A two-dimensional difference gel electrophoresis (2D-DIGE) profiling strategy was employed to examine protein expression changes between developing neuromas and normal nerves in whole tissue lysates. We found around 200 proteins which displayed a >1.75-fold change in expression between neuroma and normal nerve and identified 55 of these proteins using mass spectrometry. We also used immunoblotting to examine the expression of low-abundance ion channels Nav1.3, Nav1.8 and calcium channel alpha2delta-1 subunit in this model, since they have previously been implicated in neuronal hyperexcitability associated with neuropathic pain. Finally, S35methionine in vitro labelling of neuroma and control samples was used to demonstrate local protein synthesis of neuron-specific genes. A number of cytoskeletal proteins, enzymes and proteins associated with oxidative stress were up-regulated in neuromas, whilst overall levels of voltage-gated ion channel proteins were unaffected. We conclude that altered mRNA levels reported in the somata of damaged DRG neurons do not necessarily reflect levels of altered proteins in hyper-excitable damaged nerve endings. An altered repertoire of protein expression, local protein synthesis and topological re-arrangements of ion channels may all play important roles in neuroma hyper-excitability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.