ArticleOligonucleotides2008
Near-infrared fluorescent oligodeoxyribonucleotide reporters for sensing NF-kappaB DNA interactions in vitro.
Article in Oligonucleotides, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- NIR Fluorescent Imaging and Photodynamic Therapy with a Novel Theranostic Phospholipid Probe for Triple-Negative Breast Cancer Cells.Bioconjugate chemistry · 2021Article
- Imaging NF-κB activity in a murine model of early stage diabetes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher.ACS omega · 2018Article
- Self-neutralizing oligonucleotides with enhanced cellular uptake.Organic & biomolecular chemistry · 2017Article
- Fluorocarbons Enhance Intracellular Delivery of Short STAT3-sensors and Enable Specific Imaging.Theranostics · 2017Article
- The three-dimensional context of a double helix determines the fluorescence of the internucleoside-tethered pair of fluorophores.Molecular bioSystems · 2013Article
- Sensing of transcription factor binding via cyanine dye pair fluorescence lifetime changes.Molecular bioSystems · 2012Article
- Hairpin-like fluorescent probe for imaging of NF-κB transcription factor activity.Bioconjugate chemistry · 2011Article
- Plate capture assay of fluorescent oligonucleotide duplex reporter-transcription factor complexes.Bioconjugate chemistry · 2009Article
- In vivo investigation of breast cancer progression by use of an internal control.Neoplasia (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Two types of reporters for optical sensing of NF-kappaB p50 protein-oligodeoxyribonucleotide (ODN) duplex interactions were designed and compared in vitro. The reporters were based on the effect of fluorescence resonance energy transfer (FRET) between the pair donor Cy5.5 near-infrared (NIR) fluorochrome and either 800CW emitting fluorescence dye acceptor (800CW-Cy), or a nonemitting QSY 21 dye quencher (QSY-Cy). The donor and the acceptor dyes were covalently linked to the complementary oligonucleotides, respectively: Cy dye was conjugated to 3'-thiol, whereas 800CW or QSY21 were conjugated to a hydrophilic internucleoside phosphate amino linker. The reporters were tested initially using recombinant NF-kappaB p50 protein binding assays. Both reporters were binding p50 protein, which protected oligonucleotide duplex from degradation in the presence of exonuclease.The incubation of 800CW-Cy reporter in the presence of control or IL-1beta treated human endothelial cells showed the uptake of the reporter in the cytoplasm and the nucleus. The measurement of NIR fluorescence ratio (i.e. Cy5.5/800CW) showed a partial loss of FRET and the increased Cy5.5 fluorescence in nontreated, control cells. Thus, the specific p50 binding to ODN duplex reporters affected the donor-acceptor fluorochrome pair. NF-kappaB p50 exhibited the protective effect on FRET between NIR fluorochromes linked to the complementary strands of the reporter duplex.
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Registered trials
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