ArticleAnalytical chemistry2008
Contribution of aquaporins to cellular water transport observed by a microfluidic cell volume sensor.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Rapid Single-cell Measurement of Transient Transmembrane Water Flow under Osmotic Gradient.The Journal of membrane biology · 2026Article
- The Interplay between Meningeal Lymphatic Vessels and Neuroinflammation in Neurodegenerative Diseases.Current neuropharmacology · 2024Review
- AQP4-independent TRPV4 modulation of plasma membrane water permeability.Frontiers in cellular neuroscience · 2023Article
- Methods to Measure Water Permeability.Advances in experimental medicine and biology · 2023Article
- Molecular mechanisms of brain water transport.Nature reviews. Neuroscience · 2021Review
- The expression of endogenous voltage-gated potassium channels in HEK293 cells is affected by culture conditions.Physiological reports · 2018Article
- Weak protein-protein interactions in live cells are quantified by cell-volume modulation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Regulation and Function of AQP4 in the Central Nervous System.Neurochemical research · 2015Review
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- Shear-induced volume decrease in MDCK cells.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2012Article
- Monitoring single-channel water permeability in polarized cells.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Here we demonstrate that an impedance-based microfluidic cell volume sensor can be used to study the roles of aquaporin (AQP) in cellular water permeability and screen AQP-specific drugs. Human embryonic kidney (HEK-293) cells were transiently transfected with AQP3- or AQP4-encoding genes to express AQPs in plasma membranes. The swelling of cells in response to hypotonic stimulation was traced in real time using the sensor. Two time constants were obtained by fitting the swelling curves with a two-exponential function, a fast time constant associated with osmotic water permeability of AQP-expressing cells and a slow phase time constant associated mainly with water diffusion through lipid bilayers in the nontransfected cells. The AQP-expressing cells showed at least 10x faster osmotic water transport than control cells. Using the volume sensor, we examined the effects of Hg (2+) and Ni (2+) on the water transport via AQPs. Hg (2+) inhibited the water flux in AQP3-expressing cells irreversibly, while Ni (2+) blocked the AQP3 channels reversibly. Neither of the two ions blocked the AQP4 channels. The microfluidic volume sensor can sense changes in cell volume in real time, which enables perfusion of various reagents sequentially. It provides a convenient tool for studying the effect of reagents on the function and regulation mechanism of AQPs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.