ArticleThe Journal of biological chemistry2008
Laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the gamma1 and gamma2 chains.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Developmental Convergence Across Diverse Genetic Architectures in Childhood Apraxia of Speech.bioRxiv : the preprint server for biology · 2026Article
- Skin Deep and Beyond: Unravelling B Cell Extracellular Matrix Interactions in Cutaneous Immunity and Disease.Experimental dermatology · 2025Review
- Heparan Sulfate Chain-Conjugated Laminin-E8 Fragments Advance Paraxial Mesodermal Differentiation Followed by High Myogenic Induction from hiPSCs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Laminin N-terminus α31 expression during development is lethal and causes widespread tissue-specific defects in a transgenic mouse model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Zebrafish Models ofFrontiers in molecular neuroscience · 2020Article
- Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.Life science alliance · 2018Article
- Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells.Scientific reports · 2018Article
- Laminin-dystroglycan signaling regulates retinal arteriogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation.Cell death & disease · 2018Article
- Mechanistic basis for the recognition of laminin-511 by α6β1 integrin.Science advances · 2017Article
- Laminin-Dependent Interaction between Astrocytes and Microglia: A Role in Retinal Angiogenesis.The American journal of pathology · 2017Article
- Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo.Wellcome open research · 2017Article
- Laminin-Mediated Interactions in Thymocyte Migration and Development.Frontiers in immunology · 2015Review
- Laminins containing the β2 and γ3 chains regulate astrocyte migration and angiogenesis in the retina.Development (Cambridge, England) · 2013Article
- The γ3 chain of laminin is widely but differentially expressed in murine basement membranes: expression and functional studies.Matrix biology : journal of the International Society for Matrix Biology · 2012Article
- Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1.PloS one · 2012Article
- An integrin-binding N-terminal peptide region of TIMP-2 retains potent angio-inhibitory and anti-tumorigenic activity in vivo.Peptides · 2011Article
- Basement membranes: cell scaffoldings and signaling platforms.Cold Spring Harbor perspectives in biology · 2011Review
- Defective formation of the inner limiting membrane in laminin beta2- and gamma3-null mice produces retinal dysplasia.Investigative ophthalmology & visual science · 2010Article
- The C-terminal region of laminin beta chains modulates the integrin binding affinities of laminins.The Journal of biological chemistry · 2009Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laminins are the major cell adhesive proteins in basement membranes, and consist of three subunits termed alpha, beta, and gamma. Recently, we found that the Glu residue at the third position from the C termini of the gamma1 and gamma2 chains is critically involved in integrin binding by laminins. However, the gamma3 chain lacks this Glu residue, suggesting that laminin isoforms containing the gamma3 chain may be unable to bind to integrins. To address this possibility, we expressed the E8 fragment of laminin-213 and found that it was incapable of binding to integrins. Similarly, the E8 fragment of laminin-113 was expressed and also found to be inactive in binding to integrins, confirming the distinction between the integrin binding activities of gamma3 chain-containing isoforms and those containing the gamma1 or gamma2 chain. To further address the importance of the Glu residue, we swapped the C-terminal four amino acids of the gamma3 chain with the C-terminal nine amino acids of the gamma1 chain, which contain the Glu residue. The resulting chimeric E8 fragment of laminin-213 became fully active in integrin binding, whereas replacement with the nine amino acids of the gamma1 chain after substitution of Gln for the conserved Glu residue failed to restore the integrin binding activity. These results provide both loss-of-function and gain-of-function evidence that laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the conserved Glu residue, which should play a critical role in integrin binding by laminins.
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