ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2008
Localized application of mechanical and biochemical stimuli in 3-D culture.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- The Role of Vimentin in Human Corneal Fibroblast Spreading and Myofibroblast Transformation.Cells · 2024Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- Article
- Mechanical interactions and crosstalk between corneal keratocytes and the extracellular matrix.Experimental eye research · 2015Review
- Techniques for assessing 3-D cell-matrix mechanical interactions in vitro and in vivo.Experimental cell research · 2013Review
- Approaches to manipulating the dimensionality and physicochemical properties of common cellular scaffolds.International journal of molecular sciences · 2011Review
- Paxillin mediates sensing of physical cues and regulates directional cell motility by controlling lamellipodia positioning.PloS one · 2011Article
- Growth factor regulation of corneal keratocyte differentiation and migration in compressed collagen matrices.Investigative ophthalmology & visual science · 2010Article
- Characterization of corneal keratocyte morphology and mechanical activity within 3-D collagen matrices.Experimental eye research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The goal of this study was to investigate the responses of isolated cells in 3-D culture to localized application of mechanical and biochemical signals. Corneal fibroblasts were plated inside collagen matrices for 24 hours, then imaged using time-lapse DIC. For mechanical perturbation, a microinjection needle (Femtotip) was inserted axially into the ECM, then displaced laterally to alter local ECM stress. For biochemical stimulation, PDGF or vehicle control solution was microinjected into the matrix. Compressing the ECM perpendicular to the cell axis had no appreciable effect on cell behavior. However, pushing the ECM parallel to the cell axis induced rapid cellular contraction, followed by secondary cell spreading and tractional force generation. Injection of PDGF induced a similar cell spreading response. Cells in 3-D matrices showed remarkable plasticity, and extension of pseudopodia could be induced at both the leading and trailing edges of migrating cells.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.