ArticleCell biochemistry and biophysics2008
Force-velocity curves of motor proteins cooperating in vivo.
Article in Cell biochemistry and biophysics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Episodic transport of protein aggregates achieves a positive size selectivity in aggresome formation.Nature communications · 2025Article
- Effects of dynein inhibitor on the number of motor proteins transporting synaptic cargos.Biophysical journal · 2021Article
- Bond Type and Discretization of Nonmuscle Myosin II Are Critical for Simulated Contractile Dynamics.Biophysical journal · 2020Article
- The Impact of Rate Formulations on Stochastic Molecular Motor Dynamics.Scientific reports · 2019Article
- Investigation of multiple-dynein transport of melanosomes by non-invasive force measurement using fluctuation unit χ.Scientific reports · 2019Article
- Application of the fluctuation theorem for noninvasive force measurement in living neuronal axons.Molecular biology of the cell · 2018Article
- A Quantitative Model for BicD2/Cargo Interactions.Biochemistry · 2018Article
- Single-molecule analysis of endogenous β-actin mRNA trafficking reveals a mechanism for compartmentalized mRNA localization in axons.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cooperative protofilament switching emerges from inter-motor interference in multiple-motor transport.Scientific reports · 2014Article
- Motor coupling through lipid membranes enhances transport velocities for ensembles of myosin Va.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Force probing of individual molecules inside the living cell is now a reality.Nature chemical biology · 2012Review
- Cooperative responses of multiple kinesins to variable and constant loads.The Journal of biological chemistry · 2012Article
- Bidirectional transport by molecular motors: enhanced processivity and response to external forces.Biophysical journal · 2010Article
- Kinesin velocity increases with the number of motors pulling against viscoelastic drag.European biophysics journal : EBJ · 2010Article
- Robust transport by multiple motors with nonlinear force-velocity relations and stochastic load sharing.Physical biology · 2010Article
- A protein diffusion model of the sealing effect.Chemical engineering science · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Motor proteins convert chemical energy into work, thereby generating persistent motion of cellular and subcellular objects. The velocities of motor proteins as a function of opposing loads have been previously determined in vitro for single motors. These single molecule "force-velocity curves" have been useful for elucidating motor kinetics and for estimating motor performance under physiological loads due to, for example, the cytoplasmic drag force on transported organelles. Here we report force-velocity curves for single and multiple motors measured in vivo. Using motion enhanced differential interference contrast (MEDIC) movies of living NT2 (neuron-committed teratocarcinoma) cells at 37 degrees C, three parameters were measured--velocity (v), radius (a), and effective cytoplasmic viscosity (eta')--as they applied to moving vesicles. These parameters were combined in Stokes' equation, F = 6piaeta'v, to determine the force, F, required to transport a single intracellular particle at velocity, v. In addition, the number of active motors was inferred from the multimodal pattern seen in a normalized velocity histogram. Using this inference, the resulting in vivo force-velocity curve for a single motor agrees with previously reported in vitro single motor force-velocity curves. Interestingly, however, the curves for two and three motors lie significantly higher in both measured velocity and computed force, which suggests that motors can work cooperatively to attain higher transport forces and velocities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.