ArticlePlant molecular biology2008
Homologous recombination properties of OsRad51, a recombinase from rice.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Integrating molecular markers and phenotypic analysis to assess cold tolerance in rice germplasm.Molecular breeding : new strategies in plant improvement · 2025Article
- Natural variation of codon repeats inScience advances · 2023Article
- Application of Gamma Ray-Responsive Genes for Transcriptome-Based Phytodosimetry in Rice.Plants (Basel, Switzerland) · 2021Article
- Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants.Rice (New York, N.Y.) · 2019Review
- DNA Repair GeneInternational journal of molecular sciences · 2019Article
- Overexpression ofFrontiers in plant science · 2018Article
- Bivalent Formation 1, a plant-conserved gene, encodes an OmpH/coiled-coil motif-containing protein required for meiotic recombination in rice.Journal of experimental botany · 2017Article
- Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis.PloS one · 2017Article
- The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis.Frontiers in plant science · 2017Article
- Characterization of a plant (rice) translin and its comparative analysis with human translin.Planta · 2014Article
- Article
- Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice.The New phytologist · 2012Article
- The rice RAD51C gene is required for the meiosis of both female and male gametocytes and the DNA repair of somatic cells.Journal of experimental botany · 2012Article
- Global gene profiling of laser-captured pollen mother cells indicates molecular pathways and gene subfamilies involved in rice meiosis.Plant physiology · 2010Article
- Plant DNA recombinases: a long way to go.Journal of nucleic acids · 2010Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
cDNA corresponding to OsRad51 protein was isolated from cDNA library of rice flowers (Oryza sativa, Indica cultivar group) and cloned in to pET28a expression vector. The protein was over expressed in E. coli BL21 (DE3) and purified. Purified OsRad51 could bind single and double stranded DNA, however it showed higher affinity for single stranded DNA. Transmission Electron Microscopy (TEM) studies of OsRad51-DNA complexes showed that this protein formed ring like structures and bound DNA forming filaments. OsRad51 protein promoted renaturation of complementary single strands in to duplex DNA molecules and also showed ATPase activity, which was stimulated by single strand DNA. Fluorescence resonance energy transfer (FRET) assays revealed that OsRad51 promoted homology dependent renaturation as well as strand exchange reactions. Renaturation activity was ATP dependent; however strand exchange activity was ATP independent. This is the first report on in vitro characterization of Rad51 protein from crop plants.
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