ArticleInfection and immunity2008
Identification of a hypervariable region containing new Legionella pneumophila Icm/Dot translocated substrates by using the conserved icmQ regulatory signature.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Systematic analysis and prediction of type IV secreted effector proteins by machine learning approaches.Briefings in bioinformatics · 2019Pooled it
- Article
- TheCurrent research in microbial sciences · 2022Article
- Mitigation of Expression of Virulence Genes inPathogens (Basel, Switzerland) · 2020Article
- Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm.Microbial genomics · 2019Article
- Using an optimal set of features with a machine learning-based approach to predict effector proteins for Legionella pneumophila.PloS one · 2019Article
- RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.PLoS pathogens · 2018Article
- Article
- Exposure to synthetic gray water inhibits amoeba encystation and alters expression of Legionella pneumophila virulence genes.Applied and environmental microbiology · 2015Article
- Two Fis regulators directly repress the expression of numerous effector-encoding genes in Legionella pneumophila.Journal of bacteriology · 2014Article
- Poison domains block transit of translocated substrates via the Legionella pneumophila Icm/Dot system.Infection and immunity · 2013Article
- Cell biology of infection by Legionella pneumophila.Microbes and infection · 2013Review
- Methods for determining protein translocation by the Legionella pneumophila Dot/Icm type IV secretion system.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila.PloS one · 2011Article
- Antibiotics and UV radiation induce competence for natural transformation in Legionella pneumophila.Journal of bacteriology · 2011Article
- The E Block motif is associated with Legionella pneumophila translocated substrates.Cellular microbiology · 2011Article
- Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins.Journal of bacteriology · 2010Article
- Genome-scale identification of Legionella pneumophila effectors using a machine learning approach.PLoS pathogens · 2009Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila is an intracellular pathogen that has been shown to utilize the Icm/Dot type IV secretion system for pathogenesis. This system was shown to be composed of Icm/Dot complex components, accessory proteins, and a large number of translocated substrates. In this study, comparison of the icmQ regulatory regions from many Legionella species revealed a conserved regulatory sequence that includes the icmQ -10 promoter element. Mutagenesis of this conserved regulatory element indicated that each of the nucleotides in it affects the level of expression of the icmQ gene but not in a uniform fashion. A genomic analysis discovered that four additional genes in L. pneumophila contain this conserved regulatory sequence, which was found to function similarly in these genes as well. Examination of these four genes indicated that they are dispensable for intracellular growth, but two of them were found to encode new Icm/Dot translocated substrates (IDTS). Comparison of the genomic regions encoding these two IDTS among the four available L. pneumophila genomic sequences indicated that one of these genes is located in a hypervariable genomic region, which was shown before to contain an IDTS-encoding gene. Translocation analysis that was performed for nine proteins encoded from this hypervariable genomic region indicated that six of them are new IDTS which are translocated into host cells in an Icm/Dot-dependent manner. Furthermore, a bioinformatic analysis indicated that additional L. pneumophila genomic regions that contain several neighboring IDTS-encoding genes are hypervariable in gene content.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.