ArticleThe Journal of biological chemistry2008
Post-translational tyrosine nitration of eosinophil granule toxins mediated by eosinophil peroxidase.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Diagnostic performance of eosinopenia for identifying bacterial infections in the emergency department: a prospective multicenter study.BMC emergency medicine · 2026Article
- Article
- Non-Invasive and Minimally Invasive Biomarkers for the Management of Eosinophilic Esophagitis beyond Peak Eosinophil Counts: Filling the Gap in Clinical Practice.Diagnostics (Basel, Switzerland) · 2023Review
- Bioinformatic analysis of eosinophil activity and its implications for model and target species.Parasitology · 2020Review
- Diet-Induced Pulmonary Inflammation and Incipient Fibrosis in Mice: a Possible Role of Neutrophilic Inflammation.Inflammation · 2019Article
- White blood cell count and eosinopenia as valuable tools for the diagnosis of bacterial infections in the ED.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Observational
- Characterization of Embryo Transcriptome of Gynogenetic Olive Flounder Paralichthys olivaceus.Marine biotechnology (New York, N.Y.) · 2016Article
- Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases.The World Allergy Organization journal · 2016Review
- Simultaneous determination of 3-chlorotyrosine and 3-nitrotyrosine in human plasma by direct analysis in real time-tandem mass spectrometry.Acta pharmaceutica Sinica. B · 2015Article
- Indigenous enteric eosinophils control DCs to initiate a primary Th2 immune response in vivo.The Journal of experimental medicine · 2014Article
- Eosinophil granule proteins: form and function.The Journal of biological chemistry · 2014Review
- When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins.Journal of the American Society for Mass Spectrometry · 2012Article
- Analysing the eosinophil cationic protein--a clue to the function of the eosinophil granulocyte.Respiratory research · 2011Review
- On-line bioaffinity-electrospray mass spectrometry for simultaneous detection, identification, and quantification of protein-ligand interactions.Journal of the American Society for Mass Spectrometry · 2010Article
- Protein-peptide affinity determination using an h/d exchange dilution strategy: application to antigen-antibody interactions.Journal of the American Society for Mass Spectrometry · 2010Article
- NMR structural determinants of eosinophil cationic protein binding to membrane and heparin mimetics.Biophysical journal · 2010Article
- Biology of the eosinophil.Advances in immunology · 2009Review
- Uncommon posttranslational modifications in proteomics: ADP-ribosylation, tyrosine nitration, and tyrosine sulfation.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
21 authors at 14 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitration of tyrosine residues has been observed during various acute and chronic inflammatory diseases. However, the mechanism of tyrosine nitration and the nature of the proteins that become tyrosine nitrated during inflammation remain unclear. Here we show that eosinophils but not other cell types including neutrophils contain nitrotyrosine-positive proteins in specific granules. Furthermore, we demonstrate that the human eosinophil toxins, eosinophil peroxidase (EPO), major basic protein, eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP), and the respective murine toxins, are post-translationally modified by nitration at tyrosine residues during cell maturation. High resolution affinity-mass spectrometry identified specific single nitration sites at Tyr349 in EPO and Tyr33 in both ECP and EDN. ECP and EDN crystal structures revealed and EPO structure modeling suggested that the nitrated tyrosine residues in the toxins are surface exposed. Studies in EPO(-/-), gp91phox(-/-), and NOS(-/-) mice revealed that tyrosine nitration of these toxins is mediated by EPO in the presence of hydrogen peroxide and minute amounts of NOx. Tyrosine nitration of eosinophil granule toxins occurs during maturation of eosinophils, independent of inflammation. These results provide evidence that post-translational tyrosine nitration is unique to eosinophils.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.