ArticleThe Journal of biological chemistry2008
Biogenesis of iron-sulfur clusters in photosystem I: holo-NfuA from the cyanobacterium Synechococcus sp. PCC 7002 rapidly and efficiently transfers [4Fe-4S] clusters to apo-PsaC in vitro.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Iron-sulfur cluster biogenesis and regulation of intracellular iron homeostasis in Escherichia coli.Metallomics : integrated biometal science · 2025Review
- Novel metal sites revealed by spectroscopic and structural characterization of the ferric uptake regulator fromComputational and structural biotechnology journal · 2025Article
- The Cryptic Nature of Fe-S Clusters: A Case Study of the Hepatitis B HBx Oncoprotein.Inorganics · 2023Article
- Catalysis and Electron Transfer inChemical reviews · 2022Review
- Spectroscopic and functional characterization of the [2Fe-2S] scaffold protein Nfu from Synechocystis PCC6803.Biochimie · 2022Article
- Review
- Enzymatic and Chemical In Vitro Reconstitution of Iron-Sulfur Cluster Proteins.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Biochemical Approaches to Probe the Role of the Auxiliary Iron-Sulfur Cluster of Lipoyl Synthase from Mycobacterium Tuberculosis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Iron-Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria.Frontiers in microbiology · 2020Review
- Phosphorothioated DNA Is Shielded from Oxidative Damage.Applied and environmental microbiology · 2019Article
- Repeat proteins as versatile scaffolds for arrays of redox-active FeS clusters.Chemical communications (Cambridge, England) · 2019Article
- Presence of a [3Fe-4S] cluster in a PsaC variant as a functional component of the photosystem I electron transfer chain in Synechococcus sp. PCC 7002.Photosynthesis research · 2018Article
- Destruction and reformation of an iron-sulfur cluster during catalysis by lipoyl synthase.Science (New York, N.Y.) · 2017Article
- Recent advances in the Suf Fe-S cluster biogenesis pathway: Beyond the Proteobacteria.Biochimica et biophysica acta · 2015Review
- Nfu facilitates the maturation of iron-sulfur proteins and participates in virulence in Staphylococcus aureus.Molecular microbiology · 2015Article
- Article
- The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions.Frontiers in plant science · 2013Article
- Decreased transport restores growth of a Salmonella enterica apbC mutant on tricarballylate.Journal of bacteriology · 2012Article
- Transcriptional profiling of nitrogen fixation in Azotobacter vinelandii.Journal of bacteriology · 2011Article
- Building Fe-S proteins: bacterial strategies.Nature reviews. Microbiology · 2010Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NfuA protein has been postulated to act as a scaffolding protein in the biogenesis of photosystem (PS) I and other iron-sulfur (Fe/S) proteins in cyanobacteria and chloroplasts. To determine the properties of NfuA, recombinant NfuA from Synechococcus sp. PCC 7002 was overproduced and purified. In vitro reconstituted NfuA contained oxygen- and EDTA-labile Fe/S cluster(s), which had EPR properties consistent with [4Fe-4S] clusters. After reconstitution with 57Fe2+, Mössbauer studies of NfuA showed a broad quadrupole doublet that confirmed the presence of [4Fe-4S]2+ clusters. Native gel electrophoresis under anoxic conditions and chemical cross-linking showed that holo-NfuA forms dimers and tetramers harboring Fe/S cluster(s). Combined with iron and sulfide analyses, the results indicated that one [4Fe-4S] cluster was bound per NfuA dimer. Fe/S cluster transfer from holo-NfuA to apo-PsaC of PS I was studied by reconstitution of PS I complexes using P700-F(X) core complexes, PsaD, apo-PsaC, and holo-NfuA. Electron transfer measurements by time-resolved optical spectroscopy showed that holo-NfuA rapidly and efficiently transferred [4Fe-4S] clusters to PsaC in a reaction that required contact between the two proteins. The NfuA-reconstituted PS I complexes had typical charge recombination kinetics from [F(A)/F(B)](-) to P700+ and light-induced low-temperature EPR spectra. These results establish that cyanobacterial NfuA can act as a scaffolding protein for the insertion of [4Fe-4S] clusters into PsaC of PS I in vitro.
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