ArticleThe Journal of biological chemistry2008
Assembly of the yeast cell wall. Crh1p and Crh2p act as transglycosylases in vivo and in vitro.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 100 citations in OpenAlex.
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- Production of yeast cell wall polysaccharides-β-glucan and chitin by using food waste substrates: Biosynthesis, production, extraction, and purification methods.Comprehensive reviews in food science and food safety · 2025Review
- The "weaken-fill-repair" model for cell budding: Linking cell wall biosynthesis with mechanics.iScience · 2024Article
- Characterization ofCell surface (Amsterdam, Netherlands) · 2022Article
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- Monoclonal Antibodies Targeting Surface-Exposed Epitopes of Candida albicans Cell Wall Proteins ConferAntimicrobial agents and chemotherapy · 2022Article
- Effect of N-acetyl chito-oligosaccharides on the biosynthesis and properties of chitin in Saccharomyces cerevisiae.Folia microbiologica · 2022Article
- Fungal Pathogenesis-Related Cell Wall Biogenesis, with Emphasis on the Maize Anthracnose FungusPlants (Basel, Switzerland) · 2022Review
- The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts.Frontiers in microbiology · 2022Review
- Fungal Cell Wall Proteins and Signaling Pathways Form a Cytoprotective Network to Combat Stresses.Journal of fungi (Basel, Switzerland) · 2021Review
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- Systematic Comparison of Cell Wall-Related Proteins of Different Yeasts.Journal of fungi (Basel, Switzerland) · 2021Article
- A seven-membered cell wall related transglycosylase gene family inCell surface (Amsterdam, Netherlands) · 2020Article
- Plant Xyloglucan Xyloglucosyl Transferases and the Cell Wall Structure: Subtle but Significant.Molecules (Basel, Switzerland) · 2020Review
- Chitin and chitosan remodeling defines vegetative development and Trichoderma biocontrol.PLoS pathogens · 2020Article
- A subfamily roadmap of the evolutionarily diverse glycoside hydrolase family 16 (GH16).The Journal of biological chemistry · 2019Article
- The Glycosylphosphatidylinositol-AnchoredmSphere · 2019Article
- Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases.Nature communications · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
The cross-linking of polysaccharides to assemble new cell wall in fungi requires mechanisms by which a preexisting linkage is broken for each new one made, to allow for the absence of free energy sources outside the plasma membrane. Previous work showed that Crh1p and Crh2p, putative transglycosylases, are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan in the cell wall of budding yeast. To explore the linking reaction in vivo and in vitro, we used fluorescent sulforhodamine-linked laminari-oligosaccharides as artificial chitin acceptors. In vivo, fluorescence was detected in bud scars and at a lower level in the cell contour, both being dependent on the CRH genes. The linking reaction was also shown in digitonin-permeabilized cells, with UDP-N-acetylglucosamine as the substrate for nascent chitin production. Both the nucleotide and the Crh proteins were required here. A gas1 mutant that overexpresses Crh1p showed very high fluorescence both in intact and permeabilized cells. In the latter, fluorescence was still incorporated in patches in the absence of UDP-GlcNAc. Isolated cell walls of this strain, when incubated with sulforhodamine-oligosaccharide, also showed Crhp-dependent fluorescence in patches, which were identified as bud scars. In all three systems, binding of the fluorescent material to chitin was verified by chitinase digestion. Moreover, the cell wall reaction was inhibited by chitooligosaccharides. These results demonstrate that the Crh proteins act by transferring chitin chains to beta(1-6)glucan, with a newly observed high activity in the bud scar. The importance of transglycosylation for cell wall assembly is thus firmly established.
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