ArticleDevelopmental cell2008
A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning.
Article in Developmental cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 85 citations in OpenAlex.
- Polo-Like Kinase 1 Phosphorylation Tunes the Functional Viscoelastic Properties of the Centrosome Scaffold.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Multivalent coiled-coil interactions enable full-scale centrosome assembly and strength.The Journal of cell biology · 2024Article
- Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex.PLoS genetics · 2023Article
- CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.EMBO reports · 2021Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Going with the flow: insights fromPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Polar relaxation by dynein-mediated removal of cortical myosin II.The Journal of cell biology · 2020Article
- Regulated changes in material properties underlie centrosome disassembly during mitotic exit.The Journal of cell biology · 2020Article
- Lipid Mediated Regulation of Adult Stem Cell Behavior.Frontiers in cell and developmental biology · 2020Review
- FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning.EMBO reports · 2019Article
- Anterior-enriched filopodia create the appearance of asymmetric membrane microdomains in polarizingJournal of cell science · 2019Article
- Phosphatidylinositol-5-phosphate 4-kinase gamma accumulates at the spindle pole and prevents microtubule depolymerization.Cell division · 2019Article
- The polarity-induced force imbalance inMolecular biology of the cell · 2018Article
- Multiple Aspects of PIP2 Involvement inInternational journal of molecular sciences · 2018Article
- Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.American journal of physiology. Cell physiology · 2018Article
- Evolution of mitotic spindle behavior during the first asymmetric embryonic division of nematodes.PLoS biology · 2018Article
- Physical Limits on the Precision of Mitotic Spindle Positioning by Microtubule Pushing forces: Mechanics of mitotic spindle positioning.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- LET-99 functions in the astral furrowing pathway, where it is required for myosin enrichment in the contractile ring.Molecular biology of the cell · 2017Article
- A casein kinase 1 prevents expulsion of the oocyte meiotic spindle into a polar body by regulating cortical contractility.Molecular biology of the cell · 2017Article
- Physical determinants of asymmetric cell divisions in the early development of Caenorhabditis elegans.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Galpha regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Galpha regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.