ArticleBMC molecular biology2008
Analysis of the piggyBac transposase reveals a functional nuclear targeting signal in the 94 c-terminal residues.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- A nuclear targeting approach enables efficient transgenesis in the milkweed bugbioRxiv : the preprint server for biology · 2026Article
- Exploring transposable elements: new horizons in cancer diagnostics and therapeutics.Mobile DNA · 2025Review
- Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells.Nucleic acids research · 2022Article
- Functional Characterization of the N-Terminal Disordered Region of theInternational journal of molecular sciences · 2022Article
- Recording of DNA-binding events reveals the importance of a repurposed Candida albicans regulatory network for gut commensalism.Cell host & microbe · 2021Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- The C-terminal Domain of piggyBac Transposase Is Not Required for DNA Transposition.Journal of molecular biology · 2021Article
- Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.Nucleic acids research · 2018Article
- Preclinical and clinical advances in transposon-based gene therapy.Bioscience reports · 2017Review
- An optimized, broadly applicable piggyBac transposon induction system.Nucleic acids research · 2017Article
- TAL effectors mediate high-efficiency transposition of the piggyBac transposon in silkworm Bombyx mori L.Scientific reports · 2015Article
- PGBD5: a neural-specific intron-containing piggyBac transposase domesticated over 500 million years ago and conserved from cephalochordates to humans.Mobile DNA · 2013Article
- Size matters: versatile use of PiggyBac transposons as a genetic manipulation tool.Molecular and cellular biochemistry · 2011Review
- Nuclear importation of Mariner transposases among eukaryotes: motif requirements and homo-protein interactions.PloS one · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe piggyBac transposable element is a popular tool for germ-line transgenesis of eukaryotes. Despite this, little is known about the mechanism of transposition or the transposase (TPase) itself. A thorough understanding of just how piggyBac works may lead to more effective use of this important mobile element. A PSORTII analysis of the TPase amino acid sequence predicts a bipartite nuclear localization signal (NLS) near the c-terminus, just upstream of a putative ZnF (ZnF).
resultsWe fused the piggyBac TPase upstream of and in-frame with the enhanced yellow fluorescent protein (EYFP) in the Drosophila melanogaster inducible metallothionein protein. Using Drosophila Schneider 2 (S2) cells and the deep red fluorescent nuclear stain Draq5, we were able to track the pattern of piggyBac localization with a scanning confocal microscope 48 hours after induction with copper sulphate.
conclusionThrough n and c-terminal truncations, targeted internal deletions, and specific amino acid mutations of the piggyBac TPase open reading frame, we found that not only is the PSORTII-predicted NLS required for the TPase to enter the nucleus of S2 cells, but there are additional requirements for negatively charged amino acids a short length upstream of this region for nuclear localization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.