ArticleHuman gene therapy2008
Differential sorting of human parathyroid hormone after transduction of mouse and rat salivary glands.
Article in Human gene therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Article
- Plasmid DNA is internalized from the apical plasma membrane of the salivary gland epithelium in live animals.Histochemistry and cell biology · 2012Article
- Gene delivery in salivary glands: from the bench to the clinic.Biochimica et biophysica acta · 2011Review
- Transgenic α-1-antitrypsin secreted into the bloodstream from salivary glands is biologically active.Oral diseases · 2011Article
- α-Galactosidase A expressed in the salivary glands partially corrects organ biochemical deficits in the fabry mouse through endocrine trafficking.Human gene therapy · 2011Article
- Human parathyroid hormone is secreted primarily into the bloodstream after rat parotid gland gene transfer.Human gene therapy · 2011Article
- Salivary epithelial cells: an unassuming target site for gene therapeutics.The international journal of biochemistry & cell biology · 2010Review
- Evaluation of a rapamycin-regulated serotype 2 adeno-associated viral vector in macaque parotid glands.Oral diseases · 2010Article
- Production and sorting of transgenic, modified human parathyroid hormone in vivo in rat salivary glands.Biochemical and biophysical research communications · 2010Article
- Expression of plasmid DNA in the salivary gland epithelium: novel approaches to study dynamic cellular processes in live animals.American journal of physiology. Cell physiology · 2009Article
- In vivo veritas: the power of in situ manipulation of cells in a living animal. Focus on "Expression of plasmid DNA in the salivary gland epithelium: novel approaches to study dynamic cellular processes in live animals".American journal of physiology. Cell physiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Gene transfer to salivary glands leads to abundant secretion of transgenic protein into either saliva or the bloodstream. This indicates significant clinical potential, depending on the route of sorting. The aim of this study was to probe the sorting characteristics of human parathyroid hormone (hPTH) in two animal models for salivary gland gene transfer. PTH is a key hormone regulating calcium levels in the blood. A recombinant serotype 5 adenoviral vector carrying the hPTH cDNA was administered to the submandibular glands of mice and rats. Two days after delivery, high levels of hPTH were found in the serum of mice, leading to elevated serum calcium levels. Only low amounts of hPTH were found in the saliva. Two days after vector infusion into rats, a massive secretion of hPTH was measured in saliva, with little secretion into serum. Confocal microscopy showed hPTH in the glands, localized basolaterally in mice and apically in rats. Submandibular gland transduction was effective and the produced hPTH was biologically active in vivo. Whereas hPTH sorted toward the basolateral side in mice, in rats hPTH was secreted mainly at the apical side. These results indicate that the interaction between hPTH and the cell sorting machinery is different between mouse and rat salivary glands. Detailed studies in these two species should result in a better understanding of cellular control of transgenic secretory protein sorting in this tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.