ArticleJournal of molecular biology2008
DNA sequences in gal operon override transcription elongation blocks.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Reciprocating RNA Polymerase batters through roadblocks.Nature communications · 2024Article
- When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.Biophysical reviews · 2023Review
- Transcription of Bacterial Chromatin.Journal of molecular biology · 2019Review
- Molecular Mechanisms of Transcription Initiation at gal Promoters and their Multi-Level Regulation by GalR, CRP and DNA Loop.Biomolecules · 2015Review
- Differential role of base pairs on gal promoters strength.Journal of molecular biology · 2015Article
- Road rules for traffic on DNA-systematic analysis of transcriptional roadblocking in vivo.Nucleic acids research · 2014Article
- DNA looping-dependent autorepression of LEE1 P1 promoters by Ler in enteropathogenic Escherichia coli (EPEC).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- CodY-mediated regulation of guanosine uptake in Bacillus subtilis.Journal of bacteriology · 2011Article
- Multilevel autoregulation of λ repressor protein CI by DNA looping in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A backtrack-inducing sequence is an essential component of Escherichia coli σ(70)-dependent promoter-proximal pausing.Molecular microbiology · 2010Article
- Imprecise transcription termination within Escherichia coli greA leader gives rise to an array of short transcripts, GraL.Nucleic acids research · 2010Article
- Induction of the galactose enzymes in Escherichia coli is independent of the C-1-hydroxyl optical configuration of the inducer D-galactose.Journal of bacteriology · 2008Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
The DNA loop that represses transcription from galactose (gal) promoters is infrequently formed in stationary-phase cells because the concentration of the loop architectural protein HU is significantly low at that state, resulting in expression of the operon in the absence of the gal inducer D-galactose. Unexpectedly, transcription from the gal promoters under these conditions overrides physical block because of the presence of the Gal repressor bound to an internal operator (O(I)) located downstream of the promoters. We have shown here that although a stretch of pyrimidine residues (UUCU) in the RNA:DNA hybrid located immediately upstream of O(I) weakens the RNA:DNA hybrid and favors RNA polymerase (RNAP) pausing and backtracking, a stretch of purines (GAGAG) in the RNA present immediately upstream of the pause sequence in the hybrid acts as an antipause element by stabilizing the RNA:DNA duplex and preventing backtracking. This facilitates forward translocation of RNAP, including overriding of the DNA-bound Gal repressor barrier at O(I). When the GAGAG sequence is separated from the pyrimidine sequence by a 5-bp DNA insertion, RNAP backtracking is favored from a weak hybrid to a more stable hybrid. RNAP backtracking is sensitive to Gre factors, D-galactose, and antisense oligonucleotides. The ability of a native DNA sequence to override transcription elongation blocks in the gal operon uncovers a previously unknown way of regulating gal metabolism in Escherichia coli. It also explains the synthesis of gal enzymes in the absence of inducer for biosynthetic reactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.