ArticleAnalytical chemistry2008
Single-molecule spectroscopic study of enhanced intrinsic phycoerythrin fluorescence on silver nanostructured surfaces.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 27 citations in OpenAlex.
- Plasmon-enhanced fluorescence for biophotonics and bio-analytical applications.Frontiers in chemistry · 2024Review
- DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous PbSensors (Basel, Switzerland) · 2019Article
- Dual emission from nanoconfined R-phycoerythrin fluorescent proteins for white light emission diodes.RSC advances · 2019Article
- Silver-Gold Nanocomposite Substrates for Metal-Enhanced Fluorescence: Ensemble and Single-Molecule Spectroscopic Studies.The journal of physical chemistry. C, Nanomaterials and interfaces · 2012Article
- Distance-dependent intrinsic fluorescence of proteins on aluminum nanostructures.Proceedings of SPIE--the International Society for Optical Engineering · 2012Article
- Prism-based spectral imaging of single-molecule fluorescence from gold-nanoparticle/fluorophore complex.Journal of fluorescence · 2011Article
- Article
- Bloch Surface Wave-Coupled Emission from Quantum Dots by Ensemble and Single Molecule Spectroscopy.RSC advancesArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In this paper, we report on steady-state and time-resolved single-molecule fluorescence measurements performed on a phycobiliprotein, R-phycoerythrin (RPE), assembled on silver nanostructures. Single-molecule measurements clearly show that RPE molecules display a 10-fold increase in fluorescence intensity, with a 7-fold decrease in lifetime when they are assembled on silver nanostructured surfaces, as compared to control glass slides. The emission spectrum of individual RPE molecules also displays a significant fluorescence enhancement on silver nanostructures as compared to glass. From intensity and lifetime histograms, it is clear that the intensities as well as lifetimes of individual RPE molecules on silver nanostructures are more heterogeneously distributed than that on glass. This single-molecule study provides further insight on the heterogeneity in the fluorescence intensity and lifetimes of the RPE molecules on both glass and SiFs surfaces, which is otherwise not possible to observe using ensemble measurements. Finite-difference time-domain calculations have been performed to study the enhanced near-fields induced around silver nanoparticles by a radiating excited-state fluorophore, and the effect of such enhanced fields on the fluorescence enhancement observed is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.