ArticleAnalytical chemistry2008
Protein dynamics in iron-starved Mycobacterium tuberculosis revealed by turnover and abundance measurement using hybrid-linear ion trap-Fourier transform mass spectrometry.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Mycobacterial extracellular vesicles and host pathogen interactions.Pathogens and disease · 2018Review
- Mitochondrial Function, Biology, and Role in Disease: A Scientific Statement From the American Heart Association.Circulation research · 2016Review
- Proteome turnover in bacteria: current status for Corynebacterium glutamicum and related bacteria.Microbial biotechnology · 2013Review
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells.International journal of proteomics · 2013Article
- Article
- Protein turnover quantification in a multilabeling approach: from data calculation to evaluation.Molecular & cellular proteomics : MCP · 2012Article
- Metalloregulation of Gram-positive pathogen physiology.Current opinion in microbiology · 2012Review
- Latent tuberculosis: what the host "sees"?Immunologic research · 2011Review
- Label-free proteomics and systems biology analysis of mycobacterial phagosomes in dendritic cells and macrophages.Journal of proteome research · 2011Article
- Iron-sparing response of Mycobacterium avium subsp. paratuberculosis is strain dependent.BMC microbiology · 2010Article
- Assigning significance in label-free quantitative proteomics to include single-peptide-hit proteins with low replicates.International journal of proteomics · 2010Article
- Systems biology approaches to understanding mycobacterial survival mechanisms.Drug discovery today. Disease mechanisms · 2010Article
- Acid stress response of a mycobacterial proteome: insight from a gene ontology analysis.International journal of clinical and experimental medicine · 2009Article
- A systems biology approach to study the phagosomal proteome modulated by mycobacterial infections.International journal of clinical and experimental medicine · 2009Article
- Protein turnover in mycobacterial proteomics.Molecules (Basel, Switzerland) · 2009Review
- An assessment of false discovery rates and statistical significance in label-free quantitative proteomics with combined filters.BMC bioinformatics · 2009Article
- Principal Component Analysis of Proteome Dynamics in Iron-starved Mycobacterium Tuberculosis.Journal of proteomics & bioinformatics · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To study the proteome response of Mycobacterium tuberculosis H37Rv to a change in iron level, iron-starved late-log-phase cells were diluted in fresh low- and high-iron media containing [ (15)N]-labeled asparagine as the sole nitrogen source for labeling the proteins synthesized upon dilution. We determined the relative protein abundance and protein turnover in M. tuberculosis H37Rv under these two conditions. For measurements, we used a high-resolution hybrid-linear ion trap-Fourier transform mass spectrometer coupled with nanoliquid chromatography separation. While relative protein abundance analysis shows that only 5 proteins were upregulated by high iron, 24 proteins had elevated protein turnover for the cells in the high-iron medium. This suggests that protein turnover is a sensitive parameter to assess the proteome dynamics. Cluster analysis was used to explore the interconnection of protein abundance and turnover, revealing coordination of the cellular processes of protein synthesis, degradation, and secretion that determine the abundance and allocation of a protein in the cytosol and the extracellular matrix of the cells. Further potential utility of the approach is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.