ArticleThe American journal of tropical medicine and hygiene2008
Effects of Plasmodium falciparum mixed infections on in vitro antimalarial drug tests and genotyping.
Article in The American journal of tropical medicine and hygiene, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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Who cites it
36 citing papers in PubMed.
- Temporal multi-tissue profiling reveals metabolites linked to immune response and malaria parasitemia control.iScience · 2026Article
- Genotype Complexity Modulates Antimalarial Susceptibility in vitro.Infection and drug resistance · 2026Article
- Metabolic engineering and late-stage functionalization expand the chemical space of the antimalarial premarineosin A.Communications chemistry · 2025Article
- Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada.Emerging infectious diseases · 2022Article
- Comparative Analysis of Plasmodium falciparum Genotyping via SNP Detection, Microsatellite Profiling, and Whole-Genome Sequencing.Antimicrobial agents and chemotherapy · 2022Article
- A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses.Malaria journal · 2018Article
- A deep sequencing approach to estimate Plasmodium falciparum complexity of infection (COI) and explore apical membrane antigen 1 diversity.Malaria journal · 2017Article
- Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.Malaria journal · 2015Article
- Development of cultured Plasmodium falciparum blood-stage malaria cell banks for early phase in vivo clinical trial assessment of anti-malaria drugs and vaccines.Malaria journal · 2015Article
- Comparative analysis of field-isolate and monkey-adapted Plasmodium vivax genomes.PLoS neglected tropical diseases · 2015Article
- Multi-strain infections and 'relapse' of Leucocytozoon sabrazesi gametocytes in domestic chickens in southern China.PloS one · 2014Article
- Anti-Pfs25 human plasma reduces transmission of Plasmodium falciparum isolates that have diverse genetic backgrounds.Infection and immunity · 2013Article
- Peripheral blood stem cell transplant-related Plasmodium falciparum infection in a patient with sickle cell disease.Transfusion · 2012Article
- Longitudinal in vitro surveillance of Plasmodium falciparum sensitivity to common anti-malarials in Thailand between 1994 and 2010.Malaria journal · 2012Article
- Differential association of Plasmodium falciparum Na+/H+ exchanger polymorphism and quinine responses in field- and culture-adapted isolates of Plasmodium falciparum.Antimicrobial agents and chemotherapy · 2011Article
- Article
- Plasmodium falciparum genetic diversity maintained and amplified over 5 years of a low transmission endemic in the Peruvian Amazon.Molecular biology and evolution · 2011Article
- How much remains undetected? Probability of molecular detection of human Plasmodia in the field.PloS one · 2011Article
- An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites.Malaria journal · 2011Article
- Uncertain outcomes: adjusting for misclassification in antimalarial efficacy studies.Epidemiology and infection · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Studying drug resistance in Plasmodium falciparum requires accurate measurement of parasite response to a drug. Factors such as mixed infection of drug-resistant and -sensitive parasites can influence drug test outcome. Polymorphic DNA sequences are frequently used to detect mixed infections; infections with a single genotype or having a minor allele smaller than a subjectively selected cut-off value are often considered single infection. We studied the effects of mixed parasite populations containing various ratios of parasites resistant and sensitive to chloroquine on outcomes of drug tests and how ratios of parasite mixtures correlated with genotypes using polymerase chain reaction-based methods. Our results show that a mixture with a resistant population as low as 10% could greatly impact a drug test outcome. None of the genotyping methods could reliably detect minor DNA alleles at < or = 10%. Mixed infection presents a serious problem for drug tests, and genotyping using microsatellite or other methods may not reliably reflect true ratios of alleles.
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18689621PMC2680026What OpenQuestion holds
Registered trials
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