ArticleJournal of bacteriology2008
Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Evaluation of vector system in Saccharopolyspora erythraea and construction of new replicative vector.Applied microbiology and biotechnology · 2026Article
- A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome.Applied microbiology and biotechnology · 2026Article
- Mitigating genetic instability caused by the excision activity of theApplied and environmental microbiology · 2025Article
- Variable orthogonality of serine integrase interactions within the ϕC31 family.Scientific reports · 2024Article
- Precise Gene Knock-In Tools with Minimized Risk of DSBs: A Trend for Gene Manipulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Variable orthogonality of RDF - large serine integrase interactions within the ϕC31 family.bioRxiv : the preprint server for biology · 2024Article
- Efficient biosynthesis of transglutaminase inCurrent research in food science · 2024Article
- The influence of coiled-coil motif of serine recombinase toward the directionality regulation.Biophysical journal · 2023Article
- In vitro characterization of the site-specific recombination system based on genus Habenivirus ϕRSM small serine integrase.Molecular genetics and genomics : MGG · 2021Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Functional assembly of nitrous oxide reductase provides insights into copper site maturation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- RifZ (AMED_0655) Is a Pathway-Specific Regulator for Rifamycin Biosynthesis in Amycolatopsis mediterranei.Applied and environmental microbiology · 2017Article
- Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.Applied and environmental microbiology · 2017Article
- Engineering microbial hosts for production of bacterial natural products.Natural product reports · 2016Review
- Construction of a stepwise gene integration system by transient expression of actinophage R4 integrase in cyanobacterium Synechocystis sp. PCC 6803.Molecular genetics and genomics : MGG · 2014Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Cross-talk between diverse serine integrases.Journal of molecular biology · 2014Article
- Attachment site recognition and regulation of directionality by the serine integrases.Nucleic acids research · 2013Article
- Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Streptomyces phage phiBT1 encodes a site-specific integrase of the large serine recombinase subfamily. In this report, the enzymatic activity of the phiBT1 integrase was characterized in vitro. We showed that this integrase has efficient integration activity with substrate DNAs containing attB and attP sites, independent of DNA supercoiling or cofactors. Both intra- and intermolecular recombinations proceed with rapid kinetics. The recombination is highly specific, and no reactions are observed between pairs of sites including attB and attL, attB and attR, attP and attL, or attP and attR or between two identical att sequences; however, a low but significant frequency of excision recombination between attL and attR is observed in the presence of the phiBT1 integrase alone. In addition, for efficient integration, the minimal sizes of attB and attP are 36 bp and 48 bp, respectively. This site-specific recombination system is efficient and simple to use; thus, it could have applications for the manipulation of DNA in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.