ArticleBiophysical journal2008
Membrane mobility of beta2 integrins and rolling associated adhesion molecules in resting neutrophils.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells.Scientific reports · 2022Article
- Single-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling.Communications biology · 2021Article
- When Actin is Not Actin' Like It Should: A New Category of Distinct Primary Immunodeficiency Disorders.Journal of innate immunity · 2021Review
- Dynamics of adhesion molecule domains on neutrophil membranes: surfing the dynamic cell topography.European biophysics journal : EBJ · 2013Article
- Membrane protein dynamics and functional implications in mammalian cells.Current topics in membranes · 2013Article
- The neck region of the C-type lectin DC-SIGN regulates its surface spatiotemporal organization and virus-binding capacity on antigen-presenting cells.The Journal of biological chemistry · 2012Article
- A spatial model for integrin clustering as a result of feedback between integrin activation and integrin binding.Biophysical journal · 2012Article
- CD151 restricts the α6 integrin diffusion mode.Journal of cell science · 2012Article
- Heterogeneous diffusion of a membrane-bound pHLIP peptide.Biophysical journal · 2010Article
- Image correlation microscopy for uniform illumination.Journal of microscopy · 2010Article
- A hidden Markov model for single particle tracks quantifies dynamic interactions between LFA-1 and the actin cytoskeleton.PLoS computational biology · 2009Article
- Adhesive dynamics simulations of the mechanical shedding of L-selectin from the neutrophil surface.Journal of theoretical biology · 2009Article
- Cell adhesion molecule distribution relative to neutrophil surface topography assessed by TIRFM.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mobilities of transmembrane adhesion proteins are key underlying physical factors that contribute to neutrophil adhesion and arrest during inflammation. Here we present a novel (to our knowledge) fluorescence recovery after photobleaching system and a complementary analytical model to measure the mobility of the four key receptors involved in the adhesion cascade: L-selectin, PSGL-1, Mac-1, and LFA-1 for resting, spherical, and human neutrophils. In general, we find that beta(2) integrins (Mac-1, LFA-1) have mobilities 3-7 times faster than rolling associated molecules (L-selectin; PSGL-1), but that the mobilities within each of these groups are indistinguishable. Increasing temperature (room temperature versus 37 degrees C) results in increased mobility, in all cases, and the use of a bivalent antibody label (mAb versus Fab) decreases mobility, except in the case of rolling associated molecules at room temperature. Disrupting the actin cytoskeleton increased mobility except that the highest mobilities measured for integrins (D = 1.2 x 10(-9) cm(2)/s; 37 degrees C, Fab) are not affected by actin poisons and approach the expected value for free diffusion. Although evidence of cytoskeletal hindrance of integrin mobility has been found in other systems, our data suggest such hindrance does not limit bulk integrin diffusion in resting neutrophils over distances and times important for adhesive plaque formation.
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