ArticleNucleic acids research2008
Interactions between branched DNAs and peptide inhibitors of DNA repair.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Cationic antimicrobial peptides promote microbial mutagenesis and pathoadaptation in chronic infections.PLoS pathogens · 2014Trial
- Recent progress in probing small molecule interactions with DNA.Biophysical reviews · 2025Review
- Supramolecular Recognition of a DNA Four-Way Junction by an MAngewandte Chemie (International ed. in English) · 2025Article
- Interactions of small molecules with DNA junctions.Nucleic acids research · 2022Review
- DNA Holliday Junction: History, Regulation and Bioactivity.International journal of molecular sciences · 2022Review
- DNA folds threaten genetic stability and can be leveraged for chemotherapy.RSC chemical biology · 2021Review
- Potentiation of Antibiotics by a Novel Antimicrobial Peptide against Shiga Toxin Producing E. coli O157:H7.Scientific reports · 2020Article
- Novel antimicrobial peptide prevents C. rodentium infection in C57BL/6 mice by enhancing acid-induced pathogen killing.Microbiology (Reading, England) · 2016Article
- Hexapeptides that inhibit processing of branched DNA structures induce a dynamic ensemble of Holliday junction conformations.The Journal of biological chemistry · 2015Article
- Replication fork integrity and intra-S phase checkpoint suppress gene amplification.Nucleic acids research · 2015Article
- Antimicrobial metallopeptides with broad nuclease and ribonuclease activity.Chemical communications (Cambridge, England) · 2013Article
- wrwyrggrywrw is a single-chain functional analog of the Holliday junction-binding homodimer, (wrwycr)2.Peptides · 2013Article
- Tumor cell death mediated by peptides that recognize branched intermediates of DNA replication and repair.PloS one · 2013Article
- Similarities between exogenously- and endogenously-induced envelope stress: the effects of a new antibacterial molecule, TPI1609-10.PloS one · 2012Article
- Potent antimicrobial small molecules screened as inhibitors of tyrosine recombinases and Holliday junction-resolving enzymes.Molecular diversity · 2011Article
- A novel antimicrobial peptide significantly enhances acid-induced killing of Shiga toxin-producing Escherichia coli O157 and non-O157 serotypes.Microbiology (Reading, England) · 2011Article
- Analysis of RuvABC and RecG involvement in the escherichia coli response to the covalent topoisomerase-DNA complex.Journal of bacteriology · 2010Article
- Small molecule functional analogs of peptides that inhibit lambda site-specific recombination and bind Holliday junctions.Bioorganic & medicinal chemistry letters · 2010Article
- An antimicrobial peptide that targets DNA repair intermediates in vitro inhibits Salmonella growth within murine macrophages.Antimicrobial agents and chemotherapy · 2010Article
- Peptide wrwycr inhibits the excision of several prophages and traps holliday junctions inside bacteria.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The RecG helicase of Escherichia coli unwinds both Holliday junction (HJ) and replication fork DNA substrates. Our lab previously identified and characterized peptides (WRWYCR and KWWCRW) that block the activity of RecG on these substrates. We determined that the peptides bind HJ DNA and prevent the binding of RecG. Herein, we present further evidence that the peptides are competitive inhibitors of RecG binding to its substrates. We have generated structural models of interactions between WRWYCR and a junction substrate. Using the fluorescent probe 2-aminopurine, we show that inhibitors interact with highest affinity with HJs (K(d) = 14 nM) and approximately 4- to 9-fold more weakly with replication fork substrates. The fluorescence assay results agree with the structural model, and predict the molecular basis for interactions between HJ-trapping peptides and branched DNA molecules. Specifically, aromatic amino acids in the peptides stack with bases at the center of the DNA substrates. These interactions are stabilized by hydrogen bonds to the DNA and by intrapeptide interactions. These peptides inhibit several proteins involved in DNA repair in addition to RecG, have been useful as tools to dissect recombination, and possess antibiotic activity. Greater understanding of the peptides' mechanism of action will further increase their utility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.