ArticleThe EMBO journal1991
Functional adult acetylcholine receptor develops independently of motor innervation in Sol 8 mouse muscle cell line.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 42 citations in OpenAlex.
- Inhibition of iPLA2 β and of stretch-activated channels by doxorubicin alters dystrophic muscle function.British journal of pharmacology · 2013Article
- Functional maturation of nicotinic acetylcholine receptors as an indicator of murine muscular differentiation in a new nerve-muscle co-culture system.Pflugers Archiv : European journal of physiology · 2003Article
- Differentiation markers of mouse C2C12 and rat L6 myogenic cell lines and the effect of the differentiation medium.In vitro cellular & developmental biology. Animal · 1999Article
- Agrin can mediate acetylcholine receptor gene expression in muscle by aggregation of muscle-derived neuregulins.The Journal of cell biology · 1998Article
- Epsilon subunit-containing acetylcholine receptors in myotubes belong to the slowly degrading population.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Muscarinic Ca2+ responses resistant to muscarinic antagonists at perisynaptic Schwann cells of the frog neuromuscular junction.The Journal of physiology · 1997Article
- A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene.Molecular and cellular biology · 1995Article
- Neural regulation of muscle acetylcholine receptor epsilon- and alpha-subunit gene promoters in transgenic mice.The Journal of cell biology · 1993Article
- An 83-nucleotide promoter of the acetylcholine receptor epsilon-subunit gene confers preferential synaptic expression in mouse muscle.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We have defined culture conditions, using a feeder layer of cells from the embryonic mesenchymal cell line, 10T1/2 and a serum-free medium, which allow cells from the mouse myogenic cell line Sol 8 to form contracting myotubes for two weeks. Under these culture conditions, Sol 8 myotubes undergo a maturation process characterized by a sequential expression of two phenotypes. An early phenotype is typified by the expression of the nicotinic acetylcholine receptor (AChR) gamma-subunit transcripts and the presence of low conductance ACh-activated channels, typical of embryonic AChR. A late phenotype is characterized by the expression of AChR epsilon-subunit transcripts, the decreased accumulation of gamma-subunit transcripts and the appearance of high conductance ACh-activated channels, typical of adult AChR. These results indicate that the expression of functional adult type AChR does not require the presence of the motor nerve and therefore represents an intrinsic feature of the Sol 8 muscle cells. Chronic exposure of the cells to the voltage-sensitive Na+ channel blocking agent tetrodotoxin does not affect the appearance of the AChR epsilon-subunit transcripts but prevents the reduction of the steady-state level of the AChR gamma-subunit transcripts and yields a reduced proportion of the adult type channels. Thus, activity seems to facilitate the switch from the embryonic to the adult phenotype of the AChR protein. The Sol 8 cell system might be useful to analyse further the genetic and epigenetic regulation of muscle fibre maturation in mammals.
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