ArticleThe American journal of pathology2008
Koilocytosis: a cooperative interaction between the human papillomavirus E5 and E6 oncoproteins.
Article in The American journal of pathology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 147 citations in OpenAlex.
- Morphological and tissue-based molecular characterization of oral lesions in patients with COVID-19: A living systematic review.Archives of oral biology · 2022Pooled it
- Article
- Human papilloma virus as causal in breast cancer.Frontiers in oncology · 2026Review
- Koilocytes in the era of molecular viral detection: Still a useful cytopathic effect of human papillomavirus?CytoJournal · 2026Article
- Methodological reasons for the low prevalence of human papillomavirus infection in progressors versus non-progressors from Barrett's oesophagus to oesophageal adenocarcinoma.British journal of cancer · 2025Article
- Virus-mediated immunosuppression in head and neck cancer.Oncogene · 2025Review
- Diagnostic features of Acanthamoeba keratitis via in vivo confocal microscopy.Scientific reports · 2025Observational
- A comprehensive overview on the crosstalk between microRNAs and viral pathogenesis and infection.Medicinal research reviews · 2025Review
- HPV, APOBEC3B, and the origins of breast cancer: a narrative review and perspectives on novel mechanisms.Frontiers in oncology · 2025Review
- Tracing human papillomavirus in skin and mucosal squamous cell carcinoma: a histopathological retrospective survey.Dermatology reports · 2024Article
- Gene expression levels associated with impaired immune response and increased proliferation could serve as biomarkers for women following cervical cancer screening programmes.Frontiers in immunology · 2024Article
- CINNAMON-GUI: Revolutionizing Pap Smear Analysis with CNN-Based Digital Pathology Image Classification.F1000Research · 2024Article
- Identification of HPV oncogene and host cell differentiation associated cellular heterogeneity in cervical cancer via single-cell transcriptomic analysis.bioRxiv : the preprint server for biology · 2023Article
- Histopathological characteristics and HPV status in cervical biopsy specimens diagnosed as flat condyloma.Iranian journal of microbiology · 2023Article
- Human papillomavirus infection, cervical cancer and the less explored role of trace elements.Biological trace element research · 2023Review
- Two conserved amino acids differentiate the biology of high-risk and low-risk HPV E5 proteins.Journal of medical virology · 2022Article
- Human Papillomaviruses as Infectious Agents in Gynecological Cancers. Oncogenic Properties of Viral Proteins.International journal of molecular sciences · 2022Review
- Catching viral breast cancer.Infectious agents and cancer · 2021Review
- Assessment of Human Papillomavirus Infection and Risk Factors in Egyptian Women With Breast Cancer.Breast cancer : basic and clinical research · 2021Article
- Simultaneous circulation of COVID-19 and flu in Italy: Potential combined effects on the risk of death?International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
A long-recognized, pathognomonic feature of human papillomavirus (HPV) infection is the appearance of halo or koilocytotic cells in the differentiated layers of the squamous epithelium. These koilocytes are squamous epithelial cells that contain an acentric, hyperchromatic nucleus that is displaced by a large perinuclear vacuole. However, the genesis of the cytoplasmic vacuole has remained unclear, particularly because both HPV DNA replication and virion assembly occur exclusively in the nucleus. In clinical biopsies, koilocytosis is observed in both low- and high-risk HPV infections; therefore, in this study, we demonstrated that the E5 and E6 proteins from both low- and high-risk HPVs cooperate to induce koilocyte formation in human cervical cells in vitro, using both stable and transient assays. Both E5 and E6 also induce koilocytosis in human foreskin keratinocytes but not in primate COS cells. Deletion of the 20 C-terminal amino acids of E5 completely abrogates koilocytosis, whereas a 10-amino acid-deletion mutant retains approximately 50% of its activity. Because the E6 protein from both the low- and high-risk HPVs is capable of potentiating koilocytosis with E5, it is apparent that the targeting of both p53 and PDZ proteins by E6 is not involved. Our data suggest new, cooperative functions for both the E5 and E6 proteins, hinting at additional targets and roles for these oncoproteins in the viral life cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.