ArticleProceedings of the National Academy of Sciences of the United States of America2008
Use-dependent amplification of presynaptic Ca2+ signaling by axonal ryanodine receptors at the hippocampal mossy fiber synapse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 63 citations in OpenAlex.
- The diverse forms and roles of the neuronal endoplasmic reticulum.Nature reviews. Neuroscience · 2026Review
- Ryanodex Reduces Persistent Hippocampal Effects of Single Mild Traumatic Brain Injury in Rats.Neurotrauma reports · 2025Article
- Ectopic burst induced by blockade of axonal potassium channels on the mouse hippocampal mossy fiber.Frontiers in cellular neuroscience · 2024Article
- Article
- Potential physiological and pathological roles for axonal ryanodine receptors.Neural regeneration research · 2023Review
- Presenilins regulate synaptic plasticity in the perforant pathways of the hippocampus.Molecular brain · 2023Article
- Roles and Sources of Calcium in Synaptic Exocytosis.Advances in neurobiology · 2023Article
- Mechanistic insights into cAMP-mediated presynaptic potentiation at hippocampal mossy fiber synapses.Frontiers in cellular neuroscience · 2023Review
- Subcellular localization of hippocampal ryanodine receptor 2 and its role in neuronal excitability and memory.Communications biology · 2022Article
- Direct Ryanodine Receptor-2 Knockout in Primary Afferent Fibers Modestly Affects Neurological Recovery after Contusive Spinal Cord Injury.Neurotrauma reports · 2022Article
- Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.Neuron · 2021Article
- Fluorescence lifetime imaging reveals regulation of presynaptic CaJournal of neurochemistry · 2021Article
- Axonal Endoplasmic Reticulum Dynamics and Its Roles in Neurodegeneration.Frontiers in neuroscience · 2020Review
- Differential modulation of short-term plasticity at hippocampal mossy fiber and Schaffer collateral synapses by mitochondrial Ca2.PloS one · 2020Article
- Specific Proteomes of Hippocampal Regions CA2 and CA1 Reveal Proteins Linked to the Unique Physiology of Area CA2.Journal of proteome research · 2019Article
- Intracellular CaProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Dendritic and axonal mechanisms of Ca2+ elevation impair BDNF transport in Aβ oligomer-treated hippocampal neurons.Molecular biology of the cell · 2015Article
- Role of membrane cholesterol in spontaneous exocytosis at frog neuromuscular synapses: reactive oxygen species-calcium interplay.The Journal of physiology · 2014Article
- Interplay between synchronization of multivesicular release and recruitment of additional release sites support short-term facilitation at hippocampal mossy fiber to CA3 pyramidal cells synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Information processing and synaptic plasticity at hippocampal mossy fiber terminals.Frontiers in cellular neuroscience · 2014Review
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6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Presynaptic Ca(2+) stores have been suggested to regulate Ca(2+) dynamics within the nerve terminals at certain types of the synapse. However, little is known about their mode of activation, molecular identity, and detailed subcellular localization. Here, we show that the ryanodine-sensitive stores exist in axons and amplify presynaptic Ca(2+) accumulation at the hippocampal mossy fiber synapses, which display robust presynaptic forms of plasticity. Caffeine, a potent drug inducing Ca(2+) release from ryanodine-sensitive stores, causes elevation of presynaptic Ca(2+) levels and enhancement of transmitter release from the mossy fiber terminals. The blockers of ryanodine receptors, TMB-8 or ryanodine, reduce presynaptic Ca(2+) transients elicited by repetitive stimuli of mossy fibers but do not affect those evoked by single shocks, suggesting that ryanodine receptors amplify presynaptic Ca(2+) dynamics in an activity dependent manner. Furthermore, we generated the specific antibody against the type 2 ryanodine receptor (RyR2; originally referred to as the cardiac type) and examined the cellular and subcellular localization using immunohistochemistry. RyR2 is highly expressed in the stratum lucidum of the CA3 region and mostly colocalizes with axonal marker NF160 but not with terminal marker VGLUT1. Immunoelectron microscopy revealed that RyR2 is distributed around smooth ER within the mossy fibers but is almost excluded from their terminal portions. These results suggest that axonal localization of RyR2 at sites distant from the active zones enables use dependent Ca(2+) release from intracellular stores within the mossy fibers and thereby facilitates robust presynaptic forms of plasticity at the mossy fiber-CA3 synapse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.