ArticleThe Journal of biological chemistry2008
High affinity interaction between a bivalve C-type lectin and a biantennary complex-type N-glycan revealed by crystallography and microcalorimetry.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- C-Type Lectins from Marine Bivalves: Functional Diversity and Structural Insights.Marine drugs · 2025Pooled it
- Characterization of HOL-30: a novel tandem-repeat galectin from the marine spongeBBA advances · 2025Article
- Novel Galectins Purified from the SpongeMarine drugs · 2024Article
- A Genomic and Transcriptomic Analysis of the C-Type Lectin Gene Family Reveals Highly Expanded and Diversified Repertoires in Bivalves.Marine drugs · 2023Article
- Molecular Cloning and Characteristics of a Lectin from the BivalveMarine drugs · 2023Article
- The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata.Scientific reports · 2020Article
- A GM1b/asialo-GM1 oligosaccharide-binding R-type lectin from purplish bifurcate mussels Mytilisepta virgata and its effect on MAP kinases.The FEBS journal · 2020Article
- Functional Characterization of OXYL, A SghC1qDC LacNAc-specific Lectin from The Crinoid Feather StarMarine drugs · 2019Article
- Computational design of a symmetrical β-trefoil lectin with cancer cell binding activity.Scientific reports · 2017Article
- Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells.Glycoconjugate journal · 2017Article
- Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Mannose-Specific Lectin CGL1 from the Pacific Oyster Crassostrea gigas.Scientific reports · 2016Article
- Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types.Scientific reports · 2016Article
- A galactose-binding lectin isolated from Aplysia kurodai (sea hare) eggs inhibits streptolysin-induced hemolysis.Molecules (Basel, Switzerland) · 2014Article
- Structural analysis for glycolipid recognition by the C-type lectins Mincle and MCL.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Vibrio cholerae cytolysin recognizes the heptasaccharide core of complex N-glycans with nanomolar affinity.Journal of molecular biology · 2013Article
- A lectin from the mussel Mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells.The Journal of biological chemistry · 2012Article
- Antibody WN1 222-5 mimics Toll-like receptor 4 binding in the recognition of LPS.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A lectin from Platypodium elegans with unusual specificity and affinity for asymmetric complex N-glycans.The Journal of biological chemistry · 2012Article
- Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai.Toxins · 2012Article
- Specificity analysis of the C-type lectin from rattlesnake venom, and its selectivity towards Gal- or GalNAc-terminated glycoproteins.Glycoconjugate journal · 2011Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Codakine is an abundant 14-kDa mannose-binding C-type lectin isolated from the gills of the sea bivalve Codakia orbicularis. Binding studies using inhibition of hemagglutination indicated specificity for mannose and fucose monosaccharides. Further experiments using a glycan array demonstrated, however, a very fine specificity for N-linked biantennary complex-type glycans. An unusually high affinity was measured by titration microcalorimetry performed with a biantennary Asn-linked nonasaccharide. The crystal structure of the native lectin at 1.3A resolution revealed a new type of disulfide-bridged homodimer. Each monomer displays three intramolecular disulfide bridges and contains only one calcium ion located in the canonical binding site that is occupied by a glycerol molecule. The structure of the complex between Asn-linked nonasaccharide and codakine has been solved at 1.7A resolution. All residues could be located in the electron density map, except for the capping beta1-4-linked galactosides. The alpha1-6-linked mannose binds to calcium by coordinating the O3 and O4 hydroxyl groups. The GlcNAc moiety of the alpha1,6 arm engages in several hydrogen bonds with the protein, whereas the GlcNAc on the other antenna is stacked against Trp(108), forming an extended binding site. This is the first structural report for a bivalve lectin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.