ArticleBMC cell biology2008
Myosin-Vb functions as a dynamic tether for peripheral endocytic compartments during transferrin trafficking.
Article in BMC cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 62 citations in OpenAlex.
- Microvillus inclusion disease-associated MYO5B deficiency impairs endosome-to-mitochondrion iron transfer.Gastroenterology report · 2026Article
- Molecular Mechanisms of AMPA Receptor Trafficking in the Nervous System.International journal of molecular sciences · 2023Review
- Timing of Phagosome Maturation Depends on Their Transport Switching from Actin to Microtubule Tracks.The journal of physical chemistry. B · 2023Article
- Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS.Nature communications · 2022Article
- Prolonged ethanol exposure modulates constitutive internalization and recycling of 5-HT1A receptors.Journal of neurochemistry · 2022Article
- Multiple roles for actin in secretory and endocytic pathways.Current biology : CB · 2021Review
- High-Precision Protein-Tracking With Interferometric Scattering Microscopy.Frontiers in cell and developmental biology · 2020Article
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- Role of Alström syndrome 1 in the regulation of blood pressure and renal function.JCI insight · 2018Article
- Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication.Scientific reports · 2017Article
- Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.PLoS pathogens · 2017Article
- Clustering of Rab11 vesicles in influenza A virus infected cells creates hotspots containing the 8 viral ribonucleoproteins.Small GTPases · 2017Review
- Myosin Vb mediates Cu+ export in polarized hepatocytes.Journal of cell science · 2016Article
- Recycling Endosomes and Viral Infection.Viruses · 2016Review
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3.The Journal of cell biology · 2015Article
- Myosin VI must dimerize and deploy its unusual lever arm in order to perform its cellular roles.Cell reports · 2014Article
- The role of the cytoskeleton and molecular motors in endosomal dynamics.Seminars in cell & developmental biology · 2014Review
- Myosin Vb uncoupling from RAB8A and RAB11A elicits microvillus inclusion disease.The Journal of clinical investigation · 2014Article
- Coupling of two non-processive myosin 5c dimers enables processive stepping along actin filaments.Scientific reports · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMyosin-Vb has been shown to be involved in the recycling of diverse proteins in multiple cell types. Studies on transferrin trafficking in HeLa cells using a dominant-negative myosin-Vb tail fragment suggested that myosin-Vb was required for recycling from perinuclear compartments to the plasma membrane. However, chemical-genetic, dominant-negative experiments, in which myosin-Vb was specifically induced to bind to actin, suggested that the initial hypothesis was incorrect both in its site and mode of myosin-Vb action. Instead, the chemical-genetic data suggested that myosin-Vb functions in the actin-rich periphery as a dynamic tether on peripheral endosomes, retarding transferrin transport to perinuclear compartments.
resultsIn this study, we employed both approaches, with the addition of overexpression of full-length wild-type myosin-Vb and switching the order of myosin-Vb inhibition and transferrin loading, to distinguish between these hypotheses. Overexpression of full-length myosin-Vb produced large peripheral endosomes. Chemical-genetic inhibition of myosin-Vb after loading with transferrin did not prevent movement of transferrin from perinuclear compartments; however, virtually all myosin-Vb-decorated particles, including those moving on microtubules, were halted by the inhibition. Overexpression of the myosin-Vb tail caused a less-peripheral distribution of early endosome antigen-1 (EEA1).
conclusionAll results favored the peripheral dynamic tethering hypothesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.