ArticleMolecular cancer2008
The parafibromin tumor suppressor protein interacts with actin-binding proteins actinin-2 and actinin-3.
Article in Molecular cancer, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- The clinicopathological and prognostic significances of CDC73 expression in breast cancer: A pathological and bioinformatics analysis.Histology and histopathology · 2023Article
- Overexpression of HSF2 binding protein suppresses endoplasmic reticulum stress via regulating subcellular localization of CDC73 in hepatocytes.Cell & bioscience · 2023Article
- Tumor microenvironment-associated lactate metabolism regulates the prognosis and precise checkpoint immunotherapy outcomes of patients with lung adenocarcinoma.European journal of medical research · 2022Article
- Parafibromin governs cell polarity and centrosome assembly in Drosophila neural stem cells.PLoS biology · 2022Article
- The roles of the tumor suppressor parafibromin in cancer.Frontiers in cell and developmental biology · 2022Review
- Establishment of a novel glycolysis-related prognostic gene signature for ovarian cancer and its relationships with immune infiltration of the tumor microenvironment.Journal of translational medicine · 2021Article
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- Identification of hub methylated-CpG sites and associated genes in oral squamous cell carcinoma.Cancer medicine · 2020Article
- High Expression Levels ofJournal of Cancer · 2019Article
- The clinicopathological and prognostic significances ofOncotarget · 2017Article
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- The roles of parafibromin expression in ovarian epithelial carcinomas: a marker for differentiation and prognosis and a target for gene therapy.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- The clinicopathological significances and biological functions of parafibromin expression in head and neck squamous cell carcinomas.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- The many roles of the conserved eukaryotic Paf1 complex in regulating transcription, histone modifications, and disease states.Biochimica et biophysica acta · 2013Article
- Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype.The Journal of cell biology · 2011Article
- Clinical and molecular genetics of parathyroid neoplasms.Best practice & research. Clinical endocrinology & metabolism · 2010Review
- Article
- Hyperparathyroid genes: sequences reveal answers and questions.Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical EndocrinologistsReview
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3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundGermline and somatic inactivating mutations in the HRPT2 gene occur in the inherited hyperparathyroidism-jaw tumor syndrome, in some cases of parathyroid cancer and in some cases of familial hyperparathyroidism. HRPT2 encodes parafibromin. To identify parafibromin interacting proteins we used the yeast two-hybrid system for screening a heart cDNA library with parafibromin as the bait.
resultsFourteen parafibromin interaction positive preys representing 10 independent clones encoding actinin-2 were isolated. Parafibromin interacted with muscle alpha-actinins (actinin-2 and actinin-3), but not with non-muscle alpha-actinins (actinin-1 and actinin-4). The parafibromin-actinin interaction was verified by yeast two-hybrid, GST pull-down, and co-immunoprecipitation. Yeast two-hybrid analysis revealed that the N-terminal region of parafibromin interacted with actinins. In actin sedimentation assays parafibromin did not dissociate skeletal muscle actinins from actin filaments, but interestingly, parafibromin could also bundle/cross-link actin filaments. Parafibromin was predominantly nuclear in undifferentiated proliferating myoblasts (C2C12 cells), but in differentiated C2C12 myotubes parafibromin co-localized with actinins in the cytoplasmic compartment.
conclusionThese data support a possible contribution of parafibromin outside the nucleus through its interaction with actinins and actin bundling/cross-linking. These data also suggest that actinins (and actin) participate in sequestering parafibromin in the cytoplasmic compartment.
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