ArticleProteomics2008
Comprehensive proteomics of Methylobacterium extorquens AM1 metabolism under single carbon and nonmethylotrophic conditions.
Article in Proteomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium.BMC plant biology · 2024Article
- Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase fromInternational journal of molecular sciences · 2022Article
- EfgA is a conserved formaldehyde sensor that leads to bacterial growth arrest in response to elevated formaldehyde.PLoS biology · 2021Article
- Article
- Designing and EngineeringFrontiers in microbiology · 2019Article
- Metals and Methanotrophy.Applied and environmental microbiology · 2018Review
- AcidotolerantFrontiers in microbiology · 2017Article
- Difference in C3-C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1.BMC microbiology · 2016Article
- The One-carbon Carrier Methylofuran from Methylobacterium extorquens AM1 Contains a Large Number of α- and γ-Linked Glutamic Acid Residues.The Journal of biological chemistry · 2016Article
- Biotechnological and agronomic potential of endophytic pink-pigmented methylotrophic Methylobacterium spp.BioMed research international · 2015Review
- Proteomics of Fusobacterium nucleatum within a model developing oral microbial community.MicrobiologyOpen · 2014Article
- Plant-bacterium interactions analyzed by proteomics.Frontiers in plant science · 2013Article
- Metabolic adaptation and trophic strategies of soil bacteria-C1- metabolism and sulfur chemolithotrophy in Starkeya novella.Frontiers in microbiology · 2013Article
- Proteomics of Streptococcus gordonii within a model developing oral microbial community.BMC microbiology · 2012Article
- The ethylmalonyl-CoA pathway is used in place of the glyoxylate cycle by Methylobacterium extorquens AM1 during growth on acetate.The Journal of biological chemistry · 2012Article
- Article
- Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1.BMC systems biology · 2011Article
- An integrated proteomics/transcriptomics approach points to oxygen as the main electron sink for methanol metabolism in Methylotenera mobilis.Journal of bacteriology · 2011Article
- Article
- Streamlined pentafluorophenylpropyl column liquid chromatography-tandem quadrupole mass spectrometry and global (13)C-labeled internal standards improve performance for quantitative metabolomics in bacteria.Journal of chromatography. A · 2010Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
In order to validate a gel free quantitative proteomics assay for the model methylotrophic bacterium Methylobacterium extorquens AM1, we examined the M. extorquens AM1 proteome under single carbon (methanol) and multicarbon (succinate) growth, conditions that have been studied for decades and for which extensive corroborative data have been compiled. In total, 4447 proteins from a database containing 7556 putative ORFs from M. extorquens AM1 could be identified with two or more peptide sequences, corresponding to a qualitative proteome coverage of 58%. Statistically significant nonzero (log(2) scale) differential abundance ratios of methanol/succinate could be detected for 317 proteins using summed ion intensity measurements and 585 proteins using spectral counting, at a q-value cut-off of 0.01, a measure of false discovery rate. The results were compared to recent microarray studies performed under equivalent chemostat conditions. The M. extorquens AM1 studies demonstrated the feasibility of scaling up the multidimensional capillary HPLC MS/MS approach to a prokaryotic organism with a proteome more than three times the size of microbes we have investigated previously, while maintaining a high degree of proteome coverage and reliable quantitative abundance ratios.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.