ArticleJournal of clinical microbiology2008
Detection and serotyping of dengue virus in serum samples by multiplex reverse transcriptase PCR-ligase detection reaction assay.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 59 citations in OpenAlex.
- Fluorescence-Based Detection ofMethods and protocols · 2025Article
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- Virological Surveillance of Aedes aegypti Vectors Identifies All Four Dengue Serotypes in a Hyperendemic Region.EcoHealth · 2022Article
- A Multiplex PCR/LDR Assay for Viral Agents of Diarrhea with the Capacity to Genotype Rotavirus.Scientific reports · 2018Article
- Biographical Feature: Davise H. Larone, Ph.D.Journal of clinical microbiology · 2018Article
- Minimum InDel pattern analysis of the Zika virus.BMC genomics · 2018Article
- Cloning and Expression of NS3 Gene of Pakistani Isolate Type 2 Dengue Virus.Journal of veterinary research · 2018Article
- Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications.Mutation research. Reviews in mutation research · 2017Review
- Article
- Spatial-Temporal Co-Circulation of Dengue Virus 1, 2, 3, and 4 Associated with Coinfection Cases in a Hyperendemic Area of Brazil: A 4-Week Survey.The American journal of tropical medicine and hygiene · 2016Article
- Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine.Biomicrofluidics · 2016Review
- Article
- Universal single-probe RT-PCR assay for diagnosis of dengue virus infections.PLoS neglected tropical diseases · 2014Article
- Computer Aided Screening of Phytochemicals from Garcinia against the Dengue NS2B/NS3 Protease.Bioinformation · 2014Article
- Molecular Docking Based Screening of Plant Flavonoids as Dengue NS1 Inhibitors.Bioinformation · 2014Article
- Single-reaction, multiplex, real-time rt-PCR for the detection, quantitation, and serotyping of dengue viruses.PLoS neglected tropical diseases · 2013Article
- Fully integrated thermoplastic genosensor for the highly sensitive detection and identification of multi-drug-resistant tuberculosis.Angewandte Chemie (International ed. in English) · 2012Article
- Development of a flow-through [corrected] microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of European Bunyaviruses. [corrected].Molecular biotechnology · 2011Article
- Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan.BMC microbiology · 2011Article
- PCR/LDR/universal array platforms for the diagnosis of infectious disease.Methods in molecular biology (Clifton, N.J.) · 2010Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
The detection and successful typing of dengue virus (DENV) from patients with suspected dengue fever is important both for the diagnosis of the disease and for the implementation of epidemiologic control measures. A technique for the multiplex detection and typing of DENV serotypes 1 to 4 (DENV-1 to DENV-4) from clinical samples by PCR-ligase detection reaction (LDR) has been developed. A serotype-specific PCR amplifies the regions of genes C and E simultaneously. The two amplicons are targeted in a multiplex LDR, and the resultant fluorescently labeled ligation products are detected on a universal array. The assay was optimized using 38 DENV strains and was evaluated with 350 archived acute-phase serum samples. The sensitivity of the assay was 98.7%, and its specificity was 98.4%, relative to the results of real-time PCR. The detection threshold was 0.017 PFU for DENV-1, 0.004 PFU for DENV-2, 0.8 PFU for DENV-3, and 0.7 PFU for DENV-4. The assay is specific; it does not cross-react with the other flaviviruses tested (West Nile virus, St. Louis encephalitis virus, Japanese encephalitis virus, Kunjin virus, Murray Valley virus, Powassan virus, and yellow fever virus). All but 1 of 26 genotypic variants of DENV serotypes in a global DENV panel from different geographic regions were successfully identified. The PCR-LDR assay is a rapid, sensitive, specific, and high-throughput technique for the simultaneous detection of all four serotypes of DENV.
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Registered trials
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