ArticleJournal of virology2008
Identification of a conserved RNA replication element (cre) within the 3Dpol-coding sequence of hepatoviruses.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Identification of mutations in viral proteins involved in cell adaptation using a reverse genetic system of the live attenuated hepatitis A virus vaccine H2 strain.Virologica Sinica · 2024Article
- Identification and Genomic Characterization of Two Novel Hepatoviruses in Shrews from Yunnan Province, China.Viruses · 2024Article
- Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication.Journal of virology · 2024Article
- Hepatitis A virus infection.Nature reviews. Disease primers · 2023Review
- The ZCCHC14/TENT4 complex is required for hepatitis A virus RNA synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The 5'-end motif of Senecavirus A cDNA clone is genetically modified in 36 different ways for uncovering profiles of virus recovery.Frontiers in microbiology · 2022Article
- Pathogenicity and virulence of hepatitis A virus.Virulence · 2021Review
- A single mutation in theEmerging microbes & infections · 2021Article
- Protein Nucleotidylylation in +ssRNA Viruses.Viruses · 2021Review
- Review
- Evolutionary Origins of Enteric Hepatitis Viruses.Cold Spring Harbor perspectives in medicine · 2018Review
- Hepatitis A Virus Genome Organization and Replication Strategy.Cold Spring Harbor perspectives in medicine · 2018Review
- A Novel Marsupial Hepatitis A Virus Corroborates Complex Evolutionary Patterns Shaping the Genus Hepatovirus.Journal of virology · 2018Article
- A novel hepatovirus identified in wild woodchuck Marmota himalayana.Scientific reports · 2016Article
- Novel picornavirus in domestic rabbits (Oryctolagus cuniculus var. domestica).Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2016Article
- Evolutionary origins of hepatitis A virus in small mammals.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5'-Terminal Genomic Deletions.Journal of virology · 2015Article
- Picornavirus RNA polyadenylation by 3D(pol), the viral RNA-dependent RNA polymerase.Virus research · 2015Review
- Initiation of protein-primed picornavirus RNA synthesis.Virus research · 2015Review
- Molecular biology and inhibitors of hepatitis A virus.Medicinal research reviews · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Internally located, cis-acting RNA replication elements (cre) have been identified within the genomes of viruses representing each of the major picornavirus genera (Enterovirus, Rhinovirus, Aphthovirus, and Cardiovirus) except Hepatovirus. Previous efforts to identify a stem-loop structure with cre function in hepatitis A virus (HAV), the type species of this genus, by phylogenetic analyses or thermodynamic predictions have not succeeded. However, a region of markedly suppressed synonymous codon variability was identified in alignments of HAV sequences near the 5' end of the 3D(pol)-coding sequence of HAV, consistent with noncoding constraints imposed by an underlying RNA secondary structure. Subsequent MFOLD predictions identified a 110-nucleotide (nt) complex stem-loop in this region with a typical AAACA/G cre motif in its top loop. A potentially homologous RNA structure was identified in this region of the avian encephalitis virus genome, despite little nucleotide sequence relatedness between it and HAV. Mutations that disrupted secondary RNA structure or the AAACA/G motif, without altering the amino acid sequence of 3D(pol), ablated replication of a subgenomic HAV replicon in transfected human hepatoma cells. Replication competence could be rescued by reinsertion of the native 110-nt stem-loop structure (but not an abbreviated 45-nt stem-loop) upstream of the HAV coding sequence in the replicon. These results suggest that this stem-loop is functionally similar to cre elements of other picornaviruses and likely involved in templating VPg uridylylation as in other picornaviruses, despite its significantly larger size and lower free folding energy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.