ArticleLangmuir : the ACS journal of surfaces and colloids2008
DNA curtains and nanoscale curtain rods: high-throughput tools for single molecule imaging.
Article in Langmuir : the ACS journal of surfaces and colloids, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed.
- Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.Annual review of biophysics · 2026Review
- Single-Molecule Visualization of BLM-DNA2-Mediated DNA End Resection Using DNA Curtains.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Single-molecule DNA-flow stretching assay as a versatile hybrid tool for investigating DNA-protein interactions.BMB reports · 2025Review
- Trends in Single-Molecule Total Internal Reflection Fluorescence Imaging and Their Biological Applications with Lab-on-a-Chip Technology.Sensors (Basel, Switzerland) · 2023Review
- Single-molecule techniques to visualize and to characterize liquid-liquid phase separation and phase transition.Acta biochimica et biophysica Sinica · 2023Article
- Low cost and massively parallel force spectroscopy with fluid loading on a chip.Nature communications · 2022Article
- Article
- Molecular mechanisms ofBiophysics reports · 2021Article
- The solid and liquid states of chromatin.Epigenetics & chromatin · 2021Review
- Oriented Soft DNA Curtains for Single-Molecule Imaging.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- DNA Manipulation and Single-Molecule Imaging.Molecules (Basel, Switzerland) · 2021Review
- Single-Molecule Microscopy Meets Molecular Dynamics Simulations for Characterizing the Molecular Action of Proteins on DNA and in Liquid Condensates.Frontiers in molecular biosciences · 2021Review
- Single-Molecule Techniques to Study Chromatin.Frontiers in cell and developmental biology · 2021Review
- Single-Stranded DNA Curtains for Single-Molecule Visualization of Rad51-ssDNA Filament Dynamics.Methods in molecular biology (Clifton, N.J.) · 2021Article
- How p53 Molecules Solve the Target DNA Search Problem: A Review.International journal of molecular sciences · 2020Review
- Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone.Nature communications · 2019Article
- Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Article
- Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence Microscopy.Methods in enzymology · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Single molecule visualization of protein-DNA complexes can reveal details of reaction mechanisms and macromolecular dynamics inaccessible to traditional biochemical assays. However, these techniques are often limited by the inherent difficulty of collecting statistically relevant information from experiments explicitly designed to look at single events. New approaches that increase throughput capacity of single molecule methods have the potential for making these techniques more readily applicable to a variety of biological questions involving different types of DNA transactions. Here we show that nanofabricated chromium barriers, which are located at strategic positions on a fused silica slide otherwise coated with a supported lipid bilayer, can be used to organize DNA molecules into molecular curtains. The DNA that makes up the curtains is visualized by total internal reflection fluorescence microscopy (TIRFM) allowing simultaneous imaging of hundreds or thousands of aligned molecules. These DNA curtains present a robust experimental platform portending massively parallel data acquisition of individual protein-DNA interactions in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.