ArticlePloS one2008
Meiotic recombination hotspots of fission yeast are directed to loci that express non-coding RNA.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Metabolic stress-induced long ncRNA transcription governs the formation of meiotic DNA breaks in the fission yeast fbp1 gene.PloS one · 2024Article
- Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination HotspotBiomolecules · 2022Review
- TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation.Nature cell biology · 2021Article
- Diverse DNA Sequence Motifs Activate Meiotic Recombination Hotspots Through a Common Chromatin Remodeling Pathway.Genetics · 2019Article
- Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots.Genetics · 2017Article
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- Article
- RNA in unexpected places: long non-coding RNA functions in diverse cellular contexts.Nature reviews. Molecular cell biology · 2013Review
- Acetylated Histone H3K9 is associated with meiotic recombination hotspots, and plays a role in recombination redundantly with other factors including the H3K4 methylase Set1 in fission yeast.Nucleic acids research · 2013Article
- Protein determinants of meiotic DNA break hot spots.Molecular cell · 2013Article
- Enhancement of the gene targeting efficiency of non-conventional yeasts by increasing genetic redundancy.PloS one · 2013Article
- Nucleosomal organization of replication origins and meiotic recombination hotspots in fission yeast.The EMBO journal · 2012Article
- Review
- DNA sequence-mediated, evolutionarily rapid redistribution of meiotic recombination hotspots.Genetics · 2011Article
- Important characteristics of sequence-specific recombination hotspots in Schizosaccharomyces pombe.Genetics · 2011Article
- Meiosis: making a break for it.Current opinion in cell biology · 2010Review
- Mug28, a meiosis-specific protein of Schizosaccharomyces pombe, regulates spore wall formation.Molecular biology of the cell · 2010Article
- Discrete DNA sites regulate global distribution of meiotic recombination.Trends in genetics : TIG · 2010Article
- A positive but complex association between meiotic double-strand break hotspots and open chromatin in Saccharomyces cerevisiae.Genome research · 2009Article
- Distinct histone modifications define initiation and repair of meiotic recombination in the mouse.The EMBO journal · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPolyadenylated, mRNA-like transcripts with no coding potential are abundant in eukaryotes, but the functions of these long non-coding RNAs (ncRNAs) are enigmatic. In meiosis, Rec12 (Spo11) catalyzes the formation of dsDNA breaks (DSBs) that initiate homologous recombination. Most meiotic recombination is positioned at hotspots, but knowledge of the mechanisms is nebulous. In the fission yeast genome DSBs are located within 194 prominent peaks separated on average by 65-kbp intervals of DNA that are largely free of DSBs. METHODOLOGY/PRINCIPAL
findingsWe compared the genome-wide distribution of DSB peaks to that of polyadenylated ncRNA molecules of the prl class. DSB peaks map to ncRNA loci that may be situated within ORFs, near the boundaries of ORFs and intergenic regions, or most often within intergenic regions. Unconditional statistical tests revealed that this colocalization is non-random and robust (P<or=5.5 x 10(-8)). Furthermore, we tested and rejected the hypothesis that the ncRNA loci and DSB peaks localize preferentially, but independently, to a third entity on the chromosomes. CONCLUSIONS/SIGNIFICANCE: Meiotic DSB hotspots are directed to loci that express polyadenylated ncRNAs. This reveals an unexpected, possibly unitary mechanism for what directs meiotic recombination to hotspots. It also reveals a likely biological function for enigmatic ncRNAs. We propose specific mechanisms by which ncRNA molecules, or some aspect of RNA metabolism associated with ncRNA loci, help to position recombination protein complexes at DSB hotspots within chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.