ArticleMolecular vision2008
A reproducible and quantifiable model of choroidal neovascularization induced by VEGF A165 after subretinal adenoviral gene transfer in the rabbit.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Delivery strategies for CRISPR/Cas genome editing tool for retinal dystrophies: challenges and opportunities.Asian journal of pharmaceutical sciences · 2022Review
- A new rat model of treatment-naive quiescent choroidal neovascularization induced by human VEGF165 overexpression.Biology open · 2020Article
- Intussusceptive Vascular Remodeling Precedes Pathological Neovascularization.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Gene therapy and genome surgery in the retina.The Journal of clinical investigation · 2018Review
- Up-regulated basigin-2 in microglia induced by hypoxia promotes retinal angiogenesis.Journal of cellular and molecular medicine · 2017Article
- Efficacy and safety of conbercept as a primary treatment for choroidal neovascularization secondary to punctate inner choroidopathy.BMC ophthalmology · 2017Observational
- Two Bioactive Molecular Weight Fractions of a Conditioned Medium Enhance RPE Cell Survival on Age-Related Macular Degeneration and Aged Bruch's Membrane.Translational vision science & technology · 2016Article
- Myeloid-Derived Vascular Endothelial Growth Factor and Hypoxia-Inducible Factor Are Dispensable for Ocular Neovascularization--Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Gene Delivery by Subconjunctival Injection of Adenovirus in Rats: A Study of Local Distribution, Transgene Duration and Safety.PloS one · 2015Article
- Gene Therapies for Neovascular Age-Related Macular Degeneration.Cold Spring Harbor perspectives in medicine · 2014Review
- Animal models of age-related macular degeneration and their translatability into the clinic.Expert review of ophthalmology · 2014Article
- Animal models of age related macular degeneration.Molecular aspects of medicine · 2012Review
- Animal models of choroidal and retinal neovascularization.Progress in retinal and eye research · 2010Review
- Platelet-derived growth factor-DD targeting arrests pathological angiogenesis by modulating glycogen synthase kinase-3beta phosphorylation.The Journal of biological chemistry · 2010Article
- Blood velocity measurement in the posterior segment of the rabbit eye using combined spectral Doppler and power Doppler ultrasound.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2010Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo determine the effects of the vascular endothelial growth factor (VEGF)-A(165) delivered using a high capacity adenoviral vector (HC Ad.VEGF-A) on vascular growth and pathological changes in the rabbit eye. To combine different detection methods of VEGF-A(165) overexpression-induced neovascularization in the rabbit.
methodsHC Ad.VEGF-A(165) was constructed and injected at 5 x 10(6) infectious units (iu) into the subretinal space of rabbit eyes. Two and four weeks postinjection, the development of neovascularization and the expression of HC Ad-transduced VEGF-A(165) protein were followed up in vivo by scanning laser ophthalmoscopy, fluorescein and indocyanine green angiographies and ex vivo by electron microscopy and immunohistochemistry
resultsWe observed a choroidal neovascularization (CNV) with leakage in 83% of the rabbit eyes. Our findings present clear indications that there is a significant effect on the endothelial cells of the choriocapillaris after subretinal transduction of the retinal pigment epithelium (RPE) with VEGF-A(165) vector. The choroidal endothelial cells were activated, adherent junctions opened, and the fenestration was minimized, while the extracellular matrix localized between the RPE and the endothelium of the choriocapillaris was enlarged toward the lumen of the vessels, inducing a deep invagination of the endothelial cells into the vessel lumen. They also proliferated and formed pathological vessels in the subretinal space. Moreover,there was an increased expression of basic fibroblast growth factor and VEGF-A accompanied by macrophage stimulation, retinal edema, and photoreceptor loss.
conclusionsThis is the first model of VEGF-induced CNV in the rabbit in which the pathological events following overexpression of VEGF by RPE cells have been described in detail. Many of the features of our experimental CNV resemble those observed clinically in patients having wet age-related macular degeneration.
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18682809PMC2493026W1705739557What OpenQuestion holds
Registered trials
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