Evidence map›Paper›PMID 18682228›Full record

ArticleStructure (London, England : 1993)2008

Defining molecular and domain boundaries in the bacteriophage phi29 DNA packaging motor.

Marc C Morais, Jaya S Koti, Valorie D Bowman, Emilio Reyes-Aldrete, Dwight L Anderson, Michael G Rossmann

Abstract read
In one paragraph

Article in Structure (London, England : 1993), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed.

  1. Review
  2. Viral Genomic DNA Packaging Machinery.Sub-cellular biochemistry · 2024
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marc C MoraisDepartment of Biochemistry and Molecular Biology, 301 University Boulevard, University of Texas Medical Branch, Galveston, TX 77555-0647, USA.
Jaya S Koti
Valorie D Bowman
Emilio Reyes-Aldrete
Dwight L Anderson
Michael G Rossmann

Funding

RNA IN VIRAL DNA PACKAGINGR01GM059604 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI JARDINE, PAUL JAMES · 1999 to 2017
$5.1M
Morphogenesis of bacteriophage phi29R01DE003606 · NIDCR · UNIVERSITY OF MINNESOTA TWIN CITIES · PI GRIMES, SHELLEY · 1985 to 2008
$4.7M
MORPHOGENESIS OF BACTERIOPHAGE PHI-29R37DE003606 · NIDCR · UNIVERSITY OF MINNESOTA TWIN CITIES · PI ANDERSON, DWIGHT · 1989 to 1998
–
NIDCR NIH HHS DE-03606NIDCR NIH HHS R01 DE003606NIGMS NIH HHS GM-059604
6 · The paper itself

Abstract

Cryo-electron microscopy (cryo-EM) studies of the bacteriophage phi29 DNA packaging motor have delineated the relative positions and molecular boundaries of the 12-fold symmetric head-tail connector, the 5-fold symmetric prohead RNA (pRNA), the ATPase that provides the energy for packaging, and the procapsid. Reconstructions, assuming 5-fold symmetry, were determined for proheads with 174-base, 120-base, and 71-base pRNA; proheads lacking pRNA; proheads with ATPase bound; and proheads in which the packaging motor was missing the connector. These structures are consistent with pRNA and ATPase forming a pentameric motor component around the unique vertex of proheads. They suggest an assembly pathway for the packaging motor and a mechanism for DNA translocation into empty proheads.

Indexed as

DNA PackagingProtein ConformationBacillus PhagesCryoelectron MicroscopyModels, MolecularMolecular Motor ProteinsNucleic Acid ConformationRNA PrecursorsViral ProteinsVirus AssemblyMolecular Motor ProteinsRNA PrecursorsViral Proteins

Identifiers

PMID18682228
PMCPMC2615250

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.