ArticleStructure (London, England : 1993)2008
Structure of the E. coli DNA glycosylase AlkA bound to the ends of duplex DNA: a system for the structure determination of lesion-containing DNA.
Article in Structure (London, England : 1993), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Cellular heterogeneity in DNA alkylation repair increases population genetic plasticity.Nucleic acids research · 2021Article
- The trajectory of intrahelical lesion recognition and extrusion by the human 8-oxoguanine DNA glycosylase.Nature communications · 2020Article
- Quantifying the stability of oxidatively damaged DNA by single-molecule DNA stretching.Nucleic acids research · 2018Article
- Polymerization Dynamics of the Prophage-Encoded Actin-Like Protein AlpC Is Influenced by the DNA-Binding Adapter AlpA.Frontiers in microbiology · 2017Article
- Structural basis of damage recognition by thymine DNA glycosylase: Key roles for N-terminal residues.Nucleic acids research · 2016Article
- Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.Nucleic acids research · 2015Article
- Structural and biochemical analysis of DNA helix invasion by the bacterial 8-oxoguanine DNA glycosylase MutM.The Journal of biological chemistry · 2013Article
- Recent advances in the structural mechanisms of DNA glycosylases.Biochimica et biophysica acta · 2013Review
- Enforced presentation of an extrahelical guanine to the lesion recognition pocket of human 8-oxoguanine glycosylase, hOGG1.The Journal of biological chemistry · 2012Article
- NEIL1 binding to DNA containing 2'-fluorothymidine glycol stereoisomers and the effect of editing.Chembiochem : a European journal of chemical biology · 2012Article
- Duplex interrogation by a direct DNA repair protein in search of base damage.Nature structural & molecular biology · 2012Article
- Sequence-dependent structural variation in DNA undergoing intrahelical inspection by the DNA glycosylase MutM.The Journal of biological chemistry · 2012Article
- Strandwise translocation of a DNA glycosylase on undamaged DNA.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Hidden in plain sight: subtle effects of the 8-oxoguanine lesion on the structure, dynamics, and thermodynamics of a 15-base pair oligodeoxynucleotide duplex.Biochemistry · 2011Article
- Kinetic mechanism for the excision of hypoxanthine by Escherichia coli AlkA and evidence for binding to DNA ends.Biochemistry · 2011Article
- Article
- Stoichiometry and affinity for thymine DNA glycosylase binding to specific and nonspecific DNA.Nucleic acids research · 2011Article
- Structure of Escherichia coli AlkA in complex with undamaged DNA.The Journal of biological chemistry · 2010Article
- Structure determination of DNA methylation lesions N1-meA and N3-meC in duplex DNA using a cross-linked protein-DNA system.Nucleic acids research · 2010Article
- Entrapment and structure of an extrahelical guanine attempting to enter the active site of a bacterial DNA glycosylase, MutM.The Journal of biological chemistry · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The constant attack on DNA by endogenous and exogenous agents gives rise to nucleobase modifications that cause mutations, which can lead to cancer. Visualizing the effects of these lesions on the structure of duplex DNA is key to understanding their biologic consequences. The most definitive method of obtaining such structures, X-ray crystallography, is troublesome to employ owing to the difficulty of obtaining diffraction-quality crystals of DNA. Here, we present a crystallization system that uses a protein, the DNA glycosylase AlkA, as a scaffold to mediate the crystallization of lesion-containing duplex DNA. We demonstrate the use of this system to facilitate the rapid structure determination of DNA containing the lesion 8-oxoguanine in several different sequence contexts, and also deoxyinosine and 1,N(6)-ethenoadenine, each stabilized as the corresponding 2'-flouro analog. The structures of 8-oxoguanine provide a correct atomic-level view of this important endogenous lesion in DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.