ArticleFEMS microbiology letters2008
Site-specific integration of Streptomyces PhiC31 integrase-based vectors in the chromosome of Rhodococcus equi.
Article in FEMS microbiology letters, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Article
- High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration.Science advances · 2023Article
- Use of orthogonal serine integrases to multiplex plasmid conjugation and integration fromAccess microbiology · 2021Article
- Application of serine integrases for secondary metabolite pathway assembly inSynthetic and systems biotechnology · 2020Article
- Mycoredoxins Are Required for Redox Homeostasis and Intracellular Survival in the Actinobacterial PathogenAntioxidants (Basel, Switzerland) · 2019Article
- Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina.BMC biotechnology · 2019Article
- Monitoring Protein Secretion inFrontiers in microbiology · 2018Article
- Rescue of an intracellular avirulent Rhodococcus equi replication defect by the extracellular addition of virulence-associated protein A.The Journal of veterinary medical science · 2017Article
- Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.Applied and environmental microbiology · 2017Article
- Transcriptome reprogramming by plasmid-encoded transcriptional regulators is required for host niche adaption of a macrophage pathogen.Infection and immunity · 2015Article
- VirS, an OmpR/PhoB subfamily response regulator, is required for activation of vapA gene expression in Rhodococcus equi.BMC microbiology · 2014Article
- IcgA is a virulence factor of Rhodococcus equi that modulates intracellular growth.Infection and immunity · 2014Article
- Conjugal transfer of a virulence plasmid in the opportunistic intracellular actinomycete Rhodococcus equi.Journal of bacteriology · 2012Article
- Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).Journal of industrial microbiology & biotechnology · 2012Review
- The genome of a pathogenic rhodococcus: cooptive virulence underpinned by key gene acquisitions.PLoS genetics · 2010Article
- Characterization of the role of the pathogenicity island and vapG in the virulence of the intracellular actinomycete pathogen Rhodococcus equi.Infection and immunity · 2010Article
- A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality.Nucleic acids research · 2008Article
- Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Streptomyces PhiC31-based site-specific integration was used to transform the facultative intracellular pathogen Rhodococcus equi. The transformation efficiency of vectors incorporating the PhiC31 integrase and attP sites was comparable to that of replication plasmids using the same electroporation procedure. A single attB integration site was identified within an ORF encoding a pirin-like protein, which deviates slightly from the consensus sequence of Streptomyces attB sites. Vector integration was stably maintained in the R. equi chromosome for as many as 100 generations during unselected passage in vitro. In addition, integration does not appear to affect the replication of bacteria inside macrophages. Finally, this integration system was also used to successfully complement an R. equi mutant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.