ArticleThe Journal of biological chemistry2008
Modulation of the regulatory activity of bacterial two-component systems by SlyA.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- EruA, a Regulator of Adherent-InvasiveBiomolecules · 2026Article
- ProQ-dependent activation ofmSphere · 2024Article
- Two Additional Connections between the Transcriptional Programs Controlling Invasion and Intracellular Replication of Salmonella: HilD-SprB Positively RegulatesJournal of bacteriology · 2022Article
- DissectingJournal of bacteriology · 2021Article
- How the PhoP/PhoQ System Controls Virulence and MgMicrobiology and molecular biology reviews : MMBR · 2021Review
- Article
- Threonine Phosphorylation Fine-Tunes the Regulatory Activity of Histone-Like Nucleoid Structuring Protein inFrontiers in microbiology · 2019Article
- HilD and PhoP independently regulate the expression of grhD1, a novel gene required for Salmonella Typhimurium invasion of host cells.Scientific reports · 2018Article
- Role of a single noncoding nucleotide in the evolution of an epidemic African clade ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Characterization of SlyA in Shigella flexneri Identifies a Novel Role in Virulence.Infection and immunity · 2016Article
- Reciprocal regulation of Yersinia pestis biofilm formation and virulence by RovM and RovA.Open biology · 2016Article
- Molecular basis of Yersinia enterocolitica temperature-dependent resistance to antimicrobial peptides.Journal of bacteriology · 2012Article
- The promoter architectural landscape of the Salmonella PhoP regulon.Molecular microbiology · 2012Article
- SlyA protein activates fimB gene expression and type 1 fimbriation in Escherichia coli K-12.The Journal of biological chemistry · 2011Article
- Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis.PloS one · 2011Article
- SlyA, a MarR family transcriptional regulator, is essential for virulence in Dickeya dadantii 3937.Journal of bacteriology · 2009Article
- A dual-signal regulatory circuit activates transcription of a set of divergent operons in Salmonella typhimurium.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of the transcriptional regulator SlyA by the PhoP/PhoQ two-component system controls intracellular expression of numerous factors influencing Salmonella virulence. By dissecting the SlyA regulon using stable isotope labeling with amino acids in cell culture analysis, we found that SlyA enhances overall transcription of PhoP-activated loci. This amplification of cellular responses to Mg2+ occurs when SlyA binds to the phoPQ promoter thereby activating phoP autoregulation via a positive feedback mechanism. SlyA footprints a DNA region located one helical turn upstream of the PhoP box, which overlaps the H-NS-binding motif required for signal-dependent phoP repression in high Mg2+ conditions. Therefore, binding of SlyA likely antagonizes H-NS and facilitates the interaction of PhoP to its own promoter, subsequently activating the phoPQ operon. Establishment of this regulatory circuit allows SlyA to exert its effect on the PhoP/PhoQ system specifically in Salmonella, which may confer an additional transcriptional regulation. Thus, our results provide a molecular mechanism that determines SlyA-dependent activation of PhoP-regulated genes in modulating Salmonella virulence. Evidence from this study also suggests a function of SlyA as a mediator in signal transduction from the PhoP/PhoQ system to other bacterial two-component systems in Salmonella.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.