ArticleInfection and immunity2008
Cryptococcus neoformans enters the endolysosomal pathway of dendritic cells and is killed by lysosomal components.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 79 citations in OpenAlex.
- Dendritic Cells: Multifunctional Roles in Host Defenses toJournal of fungi (Basel, Switzerland) · 2023Review
- Innate Pulmonary Phagocytes and Their Interactions with PathogenicJournal of fungi (Basel, Switzerland) · 2023Review
- Macrophage Mediated Immunomodulation DuringFrontiers in cellular and infection microbiology · 2022Review
- Protective interaction of human phagocytic APC subsets withFrontiers in immunology · 2022Article
- Real-time visualization of phagosomal pH manipulation byFrontiers in cellular and infection microbiology · 2022Article
- Antifungal activity of dendritic cell lysosomal proteins against Cryptococcus neoformans.Scientific reports · 2021Article
- Borrelia peptidoglycan interacting Protein (BpiP) contributes to the fitness of Borrelia burgdorferi against host-derived factors and influences virulence in mouse models of Lyme disease.PLoS pathogens · 2021Article
- Transcriptional Changes in Pulmonary Phagocyte Subsets Dictate the Outcome Following Interaction With The Fungal PathogenFrontiers in immunology · 2021Article
- Dormancy in Cryptococcus neoformans: 60 years of accumulating evidence.The Journal of clinical investigation · 2020Review
- Pulmonary Macrophage and Dendritic Cell Responses toFrontiers in cellular and infection microbiology · 2020Review
- Modulation of Immune Responses by Particle Size and Shape.Frontiers in immunology · 2020Review
- Monocytes and the Host Response to Fungal Pathogens.Frontiers in cellular and infection microbiology · 2020Review
- Immunomodulatory Role of Capsular Polysaccharides Constituents ofFrontiers in medicine · 2019Review
- Harnessing Macrophages for Controlled-Release Drug Delivery: Lessons From Microbes.Frontiers in pharmacology · 2019Review
- Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.Nature communications · 2018Article
- Interactions of Cryptococcus with Dendritic Cells.Journal of fungi (Basel, Switzerland) · 2018Review
- Innate Immunity against Cryptococcus, from Recognition to Elimination.Journal of fungi (Basel, Switzerland) · 2018Review
- Innate immune evasion strategies against Cryptococcal meningitis caused byExperimental and therapeutic medicine · 2017Article
- The Fungal Cell Wall: Structure, Biosynthesis, and Function.Microbiology spectrum · 2017Review
- Induction of Broad-Spectrum Protective Immunity against DisparateFrontiers in immunology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cryptococcus neoformans is an opportunistic fungal pathogen that primarily causes disease in immunocompromised individuals. Dendritic cells (DCs) can phagocytose C. neoformans, present cryptococcal antigen, and kill C. neoformans. However, early events following C. neoformans phagocytosis by DCs are not well defined. We hypothesized that C. neoformans traffics to the endosome and the lysosome following phagocytosis by DCs and is eventually killed in the lysosome. Murine bone marrow-derived DCs (BMDCs) or human monocyte-derived DCs (HDCs) were incubated with live, encapsulated C. neoformans yeast cells and opsonizing antibody. Following incubation, DCs were intracellularly stained with antibodies against EEA1 (endosome) and LAMP-1 (late endosome/lysosome). As assessed by confocal microscopy, C. neoformans trafficked to endosomal compartments of DCs within 10 min and to lysosomal compartments within 30 min postincubation. For HDCs, the studies were repeated using complement-sufficient autologous plasma for the opsonization of C. neoformans. These data showed results similar to those for antibody opsonization, with C. neoformans localized to endosomes within 20 min and to lysosomes within 60 min postincubation. Additionally, the results of live real-time imaging studies demonstrated that C. neoformans entered lysosomal compartments within 20 min following the initiation of phagocytosis. The results of scanning and transmission electron microscopy demonstrated conventional zipper phagocytosis of C. neoformans by DCs. Finally, lysosomal extracts were purified from BMDCs and incubated with C. neoformans to determine their potential to kill C. neoformans. The extracts killed C. neoformans in a dose-dependent manner. This study shows that C. neoformans enters into endosomal and lysosomal pathways following DC phagocytosis and can be killed by lysosomal components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.