ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2008
In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it, 126 citations in OpenAlex.
- Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review.Journal of biomedical optics · 2024Pooled it
- TowardsFundamental research · 2024Review
- Article
- Photoacoustic Imaging in Biomedicine and Life Sciences.Life (Basel, Switzerland) · 2022Review
- Lymph Liquid Biopsy for Detection of Cancer Stem Cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Optical clearing for photoacoustic lympho- and angiography beyond conventional depth limitPhotoacoustics · 2020Article
- Article
- Novel near-infrared fluorescent probe for live cell imaging.Experimental and therapeutic medicine · 2020Article
- Imaging Platelet Processes and Function-Current and Emerging Approaches for ImagingFrontiers in immunology · 2020Review
- Doxorubicin Activates Ryanodine Receptors in Rat Lymphatic Muscle Cells to Attenuate Rhythmic Contractions and Lymph Flow.The Journal of pharmacology and experimental therapeutics · 2019Article
- Photoacoustic clinical imaging.Photoacoustics · 2019Review
- Bioinspired magnetic nanoparticles as multimodal photoacoustic, photothermal and photomechanical contrast agents.Scientific reports · 2019Article
- High-speed microscopy for in vivo monitoring of lymph dynamics.Journal of biophotonics · 2018Article
- Current status, pitfalls and future directions in the diagnosis and therapy of lymphatic malformation.Journal of biophotonics · 2018Review
- PhotoacousticPhotoacoustics · 2017Article
- Optoacoustic response of gold nanorods in soft phantoms using high-power diode laser assemblies at 870 and 905 nm.Biomedical optics express · 2017Article
- Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces.Journal of visualized experiments : JoVE · 2016Article
- Microparticle image velocimetry approach to flow measurements in isolated contracting lymphatic vessels.Journal of biomedical optics · 2016Article
- Dynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope.Scientific reports · 2015Article
- Gold nanoparticles for photoacoustic imaging.Nanomedicine (London, England) · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Compared with blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop in vivo lymph tests using the principles of flow cytometry. Cells in living animals were counted by laser (420-2,300 nm) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data were verified by high-speed transmission, photothermal, and fluorescent imaging. Counting of melanoma and immune-related cells in normal, apoptotic, and necrotic states in lymphatics in vivo was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of white blood cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays. Multiparameter, noninvasive, portable flow cytometer can be used for preclinical studies on animals with the potential of translation to humans for in vivo PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.