ArticleEukaryotic cell2008
Characterization of a serine proteinase mediating encystation of Acanthamoeba.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 78 citations in OpenAlex.
- Demethoxycurcumin exhibits amoebicidal activity againstCurrent research in parasitology & vector-borne diseases · 2026Article
- The effect of Legionella pneumophila infection on the encystation of Acanthamoeba castellanii.Parasites, hosts and diseases · 2026Article
- Interaction ofMicroorganisms · 2025Article
- Encystment and Excystment Processes inPathogens (Basel, Switzerland) · 2025Review
- Calcium ions in tap water may increase the adhesion ability of Acanthamoeba, potentially enhancing its cytopathic effects on corneal cells.Parasite (Paris, France) · 2025Article
- Characterization of novel extracellular proteases produced by Acanthamoeba castellanii after contact with human corneal epithelial cells and their relevance to pathogenesis.Parasites & vectors · 2024Article
- The consequences of viral infection on protists.Communications biology · 2024Review
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- Sublethal Exposure to Plasma-Activated Water Influences the Morphological Characteristics, Phagocytic Ability, and Virulence of Acanthamoeba castellanii.Acta parasitologica · 2023Article
- Exposure to sublethal concentrations of chlorine enhances the cytotoxicity of Acanthamoeba castellanii.Parasitology research · 2023Article
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Acanthamoeba castellanii exhibits intron retention during encystment.Parasitology research · 2022Article
- Curcumin effect onPeerJ · 2022Article
- RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage.Microbiology spectrum · 2021Article
- Article
- The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba.Parasites & vectors · 2020Article
- Drug Discovery againstPathogens (Basel, Switzerland) · 2020Review
- Whole Organism Model to Study Molecular Mechanisms of Differentiation and Dedifferentiation.Biology · 2020Article
- Giant virus vs amoeba: fight for supremacy.Virology journal · 2019Review
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Acanthamoeba, amphizoic protozoan parasites, are causative agents of granulomatous amoebic encephalitis and amoebic keratitis. Proteinases play a role in various biologic actions in Acanthamoeba, including host tissue destruction, pathogenesis, and digestion of phagocytosed food. Interestingly, we found that encystation of Acanthamoeba was inhibited by the serine proteinase inhibitor phenylmethanesulfonyl fluoride. In this study, we characterize a serine proteinase that is involved in mediating the encystation of Acanthamoeba. This encystation-mediating serine proteinase (EMSP) is shown to be highly expressed during encystation by real-time PCR and Western blot analysis. Chemically synthesized small interfering RNA against EMSP inhibited the expression of EMSP mRNA and significantly reduced the encystation efficiency of Acanthamoeba. An EMSP-enhanced green fluorescent protein fusion protein localized to vesicle-like structures within the amoeba. Using LysoTracker analysis, these vesicular structures were confirmed to be lysosomes. After incubation of the transfected amoeba in encystment media, small fluorescent vesicle-like structures gathered and formed ball-like structures, which were identified as colocalizing with the autophagosome. Taken together, these results indicate that EMSP plays an important role in the differentiation of Acanthamoeba by promoting autolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.