ArticleThe Plant cell2008
Arabidopsis protein disulfide isomerase-5 inhibits cysteine proteases during trafficking to vacuoles before programmed cell death of the endothelium in developing seeds.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 171 citations in OpenAlex.
- Dual localization of PDI5 couples endoplasmic reticulum proteostasis to meristem organization and meiotic fidelity in Arabidopsis.The New phytologist · 2026Article
- NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana.The New phytologist · 2026Article
- RD21-like proteases: key effector hubs in plant-pathogen interactions.Journal of experimental botany · 2025Review
- Pull the fuzes: Processing protein precursors to generate apoplastic danger signals for triggering plant immunity.Plant communications · 2024Review
- Autophagy-mediated degradation of integumentary tapetum is critical for embryo pattern formation.Nature communications · 2024Article
- Integrated Transcriptome and Proteome Analysis Reveals the Regulatory Mechanism of Root Growth by Protein Disulfide Isomerase in Arabidopsis.International journal of molecular sciences · 2024Article
- Protein disulfide isomerase-9 interacts with the lumenal region of the transmembrane endoplasmic reticulum stress sensor kinase, IRE1, to modulate the unfolded protein response inFrontiers in plant science · 2024Article
- Protein Disulfide Isomerase FgEps1 Is a Secreted Virulence Factor inJournal of fungi (Basel, Switzerland) · 2023Article
- An IRE1-proteasome system signalling cohort controls cell fate determination in unresolved proteotoxic stress of the plant endoplasmic reticulum.Nature plants · 2023Article
- Article
- QTL Mapping and a Transcriptome Integrative Analysis Uncover the Candidate Genes That Control the Cold Tolerance of Maize Introgression Lines at the Seedling Stage.International journal of molecular sciences · 2023Article
- Expression Characterization ofInternational journal of molecular sciences · 2022Article
- Interference of ArabidopsisFrontiers in plant science · 2022Article
- Proteome Changes in Stem Tissues of Sunflower Lines Inoculated with Culture Filtrate ofIranian journal of biotechnology · 2021Article
- Article
- Identification of tannic cell walls at the outer surface of the endosperm upon Arabidopsis seed coat rupture.The Plant journal : for cell and molecular biology · 2020Article
- A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.Biology · 2020Article
- A novel plant protein-disulfide isomerase participates in resistance response against the TYLCV in tomato.Planta · 2020Article
- A TMT-Based Quantitative Proteome Analysis to Elucidate the TSWV Induced Signaling Cascade in Susceptible and Resistant Cultivars ofPlants (Basel, Switzerland) · 2020Article
- The Arabidopsis Protein Disulfide Isomerase Subfamily M Isoform, PDI9, Localizes to the Endoplasmic Reticulum and Influences Pollen Viability and Proper Formation of the Pollen Exine During Heat Stress.Frontiers in plant science · 2020Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein disulfide isomerase (PDI) oxidizes, reduces, and isomerizes disulfide bonds, modulates redox responses, and chaperones proteins. The Arabidopsis thaliana genome contains 12 PDI genes, but little is known about their subcellular locations and functions. We demonstrate that PDI5 is expressed in endothelial cells about to undergo programmed cell death (PCD) in developing seeds. PDI5 interacts with three different Cys proteases in yeast two-hybrid screens. One of these traffics together with PDI5 from the endoplasmic reticulum through the Golgi to vacuoles, and its recombinant form is functionally inhibited by recombinant PDI5 in vitro. Peak PDI5 expression in endothelial cells precedes PCD, whereas decreasing PDI5 levels coincide with the onset of PCD-related cellular changes, such as enlargement and subsequent collapse of protein storage vacuoles, lytic vacuole shrinkage and degradation, and nuclear condensation and fragmentation. Loss of PDI5 function leads to premature initiation of PCD during embryogenesis and to fewer, often nonviable, seeds. We propose that PDI5 is required for proper seed development and regulates the timing of PCD by chaperoning and inhibiting Cys proteases during their trafficking to vacuoles before PCD of the endothelial cells. During this transitional phase of endothelial cell development, the protein storage vacuoles become the de facto lytic vacuoles that mediate PCD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.