ArticleJournal of bacteriology2008
A defect in menadione biosynthesis induces global changes in gene expression in Staphylococcus aureus.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 70 citations in OpenAlex.
- Biological characteristics of a menadione-dependent Staphylococcus aureus small colony variant (SCV) from bovine mastitis and heme promotion of biofilm formation.Archives of microbiology · 2026Article
- Unique structural and ligand-binding properties of theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Unique structural and ligand binding properties of thebioRxiv : the preprint server for biology · 2025Article
- Universal receptive system as a novel regulator of transcriptomic activity of Staphylococcus aureus.Microbial cell factories · 2025Article
- Deciphering the human antibody response againstFrontiers in immunology · 2023Article
- The first genomic characterization of a stable, hemin-dependent small colony variant strain ofFrontiers in microbiology · 2023Article
- Acute Infection with a Tobramycin-Induced Small Colony Variant of Staphylococcus aureus Causes Increased Inflammation in the Cystic Fibrosis Rat Lung.Infection and immunity · 2022Article
- Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants.Antibiotics (Basel, Switzerland) · 2022Article
- Biofilm and Small Colony Variants-An Update onInternational journal of molecular sciences · 2022Review
- Microbial Musings - November 2021.Microbiology (Reading, England) · 2022Article
- Article
- Heme-Dependent Siderophore Utilization Promotes Iron-Restricted Growth of the Staphylococcus aureusJournal of bacteriology · 2021Article
- The road to success of coagulase-negative staphylococci: clinical significance of small colony variants and their pathogenic role in persistent infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- A functional menadione biosynthesis pathway is required for capsule production byMicrobiology (Reading, England) · 2021Article
- Modeling of stringent-response reflects nutrient stress induced growth impairment and essential amino acids in different Staphylococcus aureus mutants.Scientific reports · 2021Article
- Article
- An integrated computational and experimental study to investigate Staphylococcus aureus metabolism.NPJ systems biology and applications · 2020Article
- Persistence ofFrontiers in microbiology · 2020Review
- The Effect of Iodine-Containing Nano-Micelles, FS-1, on Antibiotic Resistance, Gene Expression and Epigenetic Modifications in the Genome of Multidrug Resistant MRSA StrainFrontiers in microbiology · 2020Article
- Novel Research Models forFrontiers in microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both the high-resolution two-dimensional protein gel electrophoresis technique and full-genome DNA microarrays were used for identification of Staphylococcus aureus genes whose expression was changed by a mutation in menD. Because the electron transport chain is interrupted, the mutant should be unable to use oxygen and nitrate as terminal electron acceptors. Consistent with this, a mutation in menD was found to cause a gene expression pattern typically detected under anaerobic conditions in wild-type cells: proteins involved in glycolytic as well as in fermentation pathways were upregulated, whereas tricarboxylic acid (TCA) cycle enzymes were significantly downregulated. Moreover, the expression of genes encoding enzymes for nitrate respiration and the arginine deiminase pathway was strongly increased in the mutant strain. These results indicate that the menD mutant, just as the site-directed S. aureus hemB mutant, generates ATP from glucose or fructose mainly by substrate phosphorylation and might be defective in utilizing a variety of carbon sources, including TCA cycle intermediates and compounds that generate ATP only via electron transport phosphorylation. Of particular interest is that there are also differences in the gene expression patterns between hemB and menD mutants. While some anaerobically active enzymes were present in equal amounts in both strains (Ldh1, SACOL2535), other classically anaerobic enzymes seem to be present in higher amounts either in the hemB mutant (e.g., PflB, Ald1, IlvA1) or in the menD mutant (arc operon). Only genes involved in nitrate respiration and the ald1 operon seem to be additionally regulated by a depletion of oxygen in the hemB and/or menD mutant.
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Registered trials
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