ArticleClinical immunology (Orlando, Fla.)2008
Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies.
Article in Clinical immunology (Orlando, Fla.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed.
- Autoantigen-specific regulatory T cells induced in patients with type 1 diabetes mellitus by insulin B-chain immunotherapy.Journal of autoimmunity · 2010Trial
- Cell-Based Immuno-Biosensors Using Microfluidics.Sensors (Basel, Switzerland) · 2026Review
- Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- IL-2 secretion-based sorting of single T cells using high-throughput microfluidic on-cell cytokine capture.Lab on a chip · 2022Article
- Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models.Sensors (Basel, Switzerland) · 2022Review
- Review
- Deep Profiling of Cellular Heterogeneity by Emerging Single-Cell Proteomic Technologies.Proteomics · 2020Review
- A Microfluidic Fluorescent Flow Cytometry Capable of Quantifying Cell Sizes and Numbers of Specific Cytosolic Proteins.Scientific reports · 2018Article
- Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research.International journal of molecular sciences · 2017Review
- Microchip-based single-cell functional proteomics for biomedical applications.Lab on a chip · 2017Review
- Immune cell profiling in cancer: molecular approaches to cell-specific identification.NPJ precision oncology · 2017Review
- Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors.Microsystems & nanoengineering · 2017Article
- Article
- Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.Sensors (Basel, Switzerland) · 2016Review
- Protein adsorption in microengraving immunoassays.Sensors (Basel, Switzerland) · 2015Article
- Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding.Analytical chemistry · 2014Article
- Single-cell technologies for monitoring immune systems.Nature immunology · 2014Review
- Detection and isolation of auto-reactive human antibodies from primary B cells.Methods (San Diego, Calif.) · 2013Article
- Immunological biomarkers: catalysts for translational advances in autoimmune diabetes.Clinical and experimental immunology · 2013Article
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7 authors.
Funding
Abstract
Cell surface determinants, cytokines and antibodies secreted by hematopoietic cells are used to classify their lineage and function. Currently available techniques are unable to elucidate multiple secreted proteins while also assigning phenotypic surface-displayed markers to the individual living cells. Here, a soft lithographic method, microengraving, was adapted for the multiplexed interrogation of populations of individual human peripheral blood mononuclear cells for secreted cytokines (IFN-gamma and IL-6), antigen-specific antibodies, and lineage-specific surface-expressed markers. Application of the method to a clinical sample from a recent-onset Type 1 diabetic subject with a positive titer of anti-insulin antibodies showed that approximately 0.58% of circulating CD19(+) B cells secreted proinsulin-reactive antibodies of the IgG isotype and 2-3% of circulating cells secreted IL-6. These data demonstrate the utility of microengraving for interrogating multiple phenotypes of single human cells concurrently and for detecting rare populations of cells by their secreted products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.