ArticleJournal of neuroscience methods2008
Closing the phenotypic gap between transformed neuronal cell lines in culture and untransformed neurons.
Article in Journal of neuroscience methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- A new advanced cellular model of functional cholinergic-like neurons developed by reprogramming the human SH-SY5Y neuroblastoma cell line.Cell death discovery · 2024Article
- Three-Dimensional Cell Cultures: The Bridge between In Vitro and In Vivo Models.International journal of molecular sciences · 2023Review
- Electrophysiological Recordings from Embryonic Mouse Motoneurons Cultured on Electrospun Poly-Lactic Acid (PLA) and Polypyrrole-Coated PLA Scaffolds.Iranian biomedical journal · 2022Article
- The neurovascular unit in leukodystrophies: towards solving the puzzle.Fluids and barriers of the CNS · 2022Review
- 3D Oral and Cervical Tissue Models for Studying Papillomavirus Host-Pathogen Interactions.Current protocols in microbiology · 2020Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
- Differential cytotoxic effects of 7-dehydrocholesterol-derived oxysterols on cultured retina-derived cells: Dependence on sterol structure, cell type, and density.Experimental eye research · 2016Article
- Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro.Annals of biomedical engineering · 2015Article
- Ethyl acetate extract of germinated brown rice attenuates hydrogen peroxide-induced oxidative stress in human SH-SY5Y neuroblastoma cells: role of anti-apoptotic, pro-survival and antioxidant genes.BMC complementary and alternative medicine · 2013Article
- Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.Neurogenetics · 2013Article
- Rotating cell culture systems for human cell culture: human trophoblast cells as a model.Journal of visualized experiments : JoVE · 2012Article
- PEP-1-frataxin significantly increases cell proliferation and neuroblast differentiation by reducing lipid peroxidation in the mouse dentate gyrus.Neurochemical research · 2011Article
- Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.Nature reviews. Microbiology · 2010Review
- Development and characterization of a three-dimensional organotypic human vaginal epithelial cell model.Biology of reproduction · 2010Article
- Microglia are mediators of Borrelia burgdorferi-induced apoptosis in SH-SY5Y neuronal cells.PLoS pathogens · 2009Article
- Decreased neuronal autophagy in HIV dementia: a mechanism of indirect neurotoxicity.Autophagy · 2008Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Studies of neuronal dysfunction in the central nervous system (CNS) are frequently limited by the failure of primary neurons to propagate in vitro. Neuronal cell lines can be substituted for primary cells but they often misrepresent normal conditions. We hypothesized that a three-dimensional (3D) cell culture system would drive the phenotype of transformed neurons closer to that of untransformed cells, as has been demonstrated in non-neuronal cell lines. In our studies comparing 3D versus two-dimensional (2D) culture, neuronal SH-SY5Y (SY) cells underwent distinct morphological changes combined with a significant drop in their rate of cell division. Expression of the proto-oncogene N-myc and the RNA-binding protein HuD was decreased in 3D culture as compared to standard 2D conditions. We observed a decline in the anti-apoptotic protein Bcl-2 in 3D culture, coupled with increased expression of the pro-apoptotic proteins Bax and Bak. Moreover, thapsigargin (TG)-induced apoptosis was enhanced in the 3D cells. Microarray analysis demonstrated significantly differing mRNA levels for over 700 genes in the cells of the two culture types, and indicated that alterations in the G1/S cell-cycle progression contributed to the diminished doubling rate in the 3D-cultured SY cells. These results demonstrate that a 3D culture approach narrows the phenotypic gap between neuronal cell lines and primary neurons. The resulting cells may readily be used for in vitro research of neuronal pathogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.