ArticleThe Journal of comparative neurology2008
Probing neurochemical structure and function of retinal ON bipolar cells with a transgenic mouse.
Article in The Journal of comparative neurology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 65 citations in OpenAlex.
- Optogenetic vision restoration in the face of secondary and tertiary remodeling in the rd1 mouse retina.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Latent epigenetic programs in Müller glia contribute to stress and disease response in the retina.Developmental cell · 2025Article
- System biology analysis reveals that Grm6 is associated with glutamate accumulation-induced scotopic vision impairment in diabetic mice.Molecular vision · 2025Article
- Intravitreal injection of a rationally designed AAV capsid library in non-human primate identifies variants with enhanced retinal transduction and neutralizing antibody evasion.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Fundus autofluorescence, optical coherence tomography and electroretinography abnormalities in a patient with digoxin retinopathy that resemble those in KCNV2-associated retinopathy.Documenta ophthalmologica. Advances in ophthalmology · 2023Article
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- Identification of a modular super-enhancer in murine retinal development.Nature communications · 2022Article
- High-resolution light-field microscopy with patterned illumination.Biomedical optics express · 2021Article
- Rescue of Defective Electroretinographic Responses in Dp71-Null Mice With AAV-Mediated Reexpression of Dp71.Investigative ophthalmology & visual science · 2020Article
- Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells.The Journal of comparative neurology · 2019Article
- Retinal Cell Type DNA Methylation and Histone Modifications Predict Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures.Cell reports · 2018Article
- The Transduction Cascade in Retinal ON-Bipolar Cells: Signal Processing and Disease.Annual review of vision science · 2017Review
- Meclofenamic acid improves the signal to noise ratio for visual responses produced by ectopic expression of human rod opsin.Molecular vision · 2017Article
- Ectopic transgene expression in the retina of four transgenic mouse lines.Brain structure & function · 2016Article
- AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents and primates.Gene therapy · 2016Article
- Novel Methodology for Creating Macaque Retinas with Sortable Photoreceptors and Ganglion Cells.Frontiers in neuroscience · 2016Article
- Differential function of Gγ13 in rod bipolar and ON cone bipolar cells.The Journal of physiology · 2015Article
- Marking cells with infrared fluorescent proteins to preserve photoresponsiveness in the retina.BioTechniques · 2014Article
- Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno-associated virus capsid and promoter.EMBO molecular medicine · 2014Article
- Localization of Cacna1s to ON bipolar dendritic tips requires mGluR6-related cascade elements.Investigative ophthalmology & visual science · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Retinal ON bipolar cells make up about 70% of all bipolar cells. Glutamate hyperpolarizes these cells by binding to the metabotropic glutamate receptor mGluR6, activating the G-protein G(o1), and closing an unidentified cation channel. To facilitate investigation of ON bipolar cells, we here report on the production of a transgenic mouse (Grm6-GFP) in which enhanced green fluorescent protein (EGFP), under control of mGluR6 promoter, was expressed in all and only ON bipolar cells. We used the mouse to determine density of ON bipolar cells, which in central retina was 29,600 cells/mm(2). We further sorted the fluorescent cells and created a pure ON bipolar cDNA library that was negative for photoreceptor unique genes. With this library, we determined expression of 27 genes of interest. We obtained positive transcripts for G(o) interactors: regulators of G-protein signaling (RGS), Ret-RGS1 (a variant of RGS20), RGS16, RGS7, purkinje cell protein 2 (PCP2, also called L7 or GPSM4), synembryn (RIC-8), LGN (GPSM2), RAP1GAP, and Gbeta5; cGMP modulators: guanylyl cyclase (GC) 1alpha1, GC1beta1, phosphodiesterase (PDE) 1C, and PDE9A; and channels: inwardly rectifying potassium channel Kir2.4, transient receptor potential TRPC2, and sperm-specific cation channels CatSper 2-4. The following transcripts were not found in our library: AGS3 (GPSM1), RGS10, RGS19 (GAIP), calbindin, GC1alpha2, GC1beta2, PDE5, PDE2A, amiloride-sensitive sodium channel ACCN4, and CatSper1. We then localized Kir2.4 to several cell types and showed that, in ON bipolar cells, the channel concentrates in their dendritic tips. The channels and modulators found in ON bipolar cells likely shape their light response. Additional uses of the Grm6-GFP mouse are also discussed.
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Registered trials
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