Evidence map›Paper›PMID 18668646›Full record

ArticleLiver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society2008

The membrane attack complex (C5b-9) in liver cold ischemia and reperfusion injury.

Constantino Fondevila, Xiu-Da Shen, Seiichiro Tsuchihashi, Yoichiro Uchida, Maria Cecilia Freitas, Bibo Ke, Ronald W Busuttil, Jerzy W Kupiec-Weglinski

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 57 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. Inflammatory response to the ischaemia-reperfusion insult in the liver after major tissue trauma.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022
    Review
  8. Review
  9. Review
  10. How Machine Perfusion Ameliorates Hepatic Ischaemia Reperfusion Injury.International journal of molecular sciences · 2021
    Review
  11. Article
  12. Review
  13. Dihydro-stilbene gigantol relieves CClActa pharmacologica Sinica · 2020
    Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 3 countries.

Constantino FondevilaDumont-University of California at Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, David Geffen School of Medicine at the University of California at Los Angeles, Los Angeles, CA 90095, USA.
Xiu-Da Shen
Seiichiro Tsuchihashi
Yoichiro Uchida
Maria Cecilia Freitas
Bibo Ke
Ronald W Busuttil
Jerzy W Kupiec-Weglinski
University of California, Los Angeles · USTCL (China) · CN

Funding

NRF2 - SIRT1 SIGNALING AXIS IN LIVER TRANSPLANT REJUVENATIONR01DK062357 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KUPIEC-WEGLINSKI, JERZY W · 2003 to 2025
$8.4M
INFECTIOUS TOLERANCE IN TRANSPLANT RECIPIENTSR01AI042223 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KUPIEC-WEGLINSKI, JERZY W · 1998 to 2008
$2.4M
NOVEL THERAPIES FOR GRAFT REJECTION IN SENSITIZED HOSTSR01AI023847 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KUPIEC-WEGLINSKI, JERZY W. · 1988 to 2006
$1.8M
COSTIMULATORY PATHWAYS IN SENSITIZED GRAFT RECIPIENTSR56AI023847 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KUPIEC-WEGLINSKI, JERZY W · 2009 to 2009
$385k
COSTIMULATORY PATHWAYS SENSITIZED GRAFT RECIPIENTSR21AI023847 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KUPIEC-WEGLINSKI, JERZY W. · 2001 to 2001
$306k
NIAID NIH HHS AI23847NIAID NIH HHS AI42223NIAID NIH HHS R01 AI023847NIAID NIH HHS R01 AI042223NIAID NIH HHS R21 AI023847NIAID NIH HHS R56 AI023847NIDDK NIH HHS R01 DK062357
6 · The paper itself

Abstract

Activation of the complement cascade represents an important event during ischemia/reperfusion injury (IRI). This work was designed to investigate the role of the membrane attack complex (MAC; C5b-9) in the pathogenesis of hepatic IRI. Livers from B&W/Stahl/rC6(+) and C6(-) rats were harvested, stored for 24 hours at 4 degrees C, and then transplanted [orthotopic liver transplantation (OLT)] to syngeneic recipients. There were 4 experimental groups: (1) C6(+)-->C6(+), (2) C6(+)-->C6(-), (3) C6(-)-->C6(+), and (4) C6(-)-->C6(-). At day +1, C6(-) OLTs showed decreased vascular congestion/necrosis, contrasting with extensive necrosis in C6(+) livers, that was independent of the recipient C6 status (Suzuki score: 7.2 +/- 0.9, 7.3 +/- 1.3, 4.5 +/- 0.6, and 4.8 +/- 0.4 for groups 1-4, respectively, P < 0.05). The liver function improved in recipients of C6(-) grafts (serum glutamic oxaloacetic transaminase: 2573 +/- 488, 1808 +/- 302, 1170 +/- 111, and 1188 +/- 184 in groups 1-4, respectively, P < 0.05). Intragraft macrophage infiltration (ED-1 immunostaining) and neutrophil infiltration (myeloperoxidase activity) were reduced in C6(-) grafts versus C6(+) grafts (P = 0.001); these data were confirmed by esterase staining (naphthol). The expression of proinflammatory interferon-gamma, interleukin-1beta, and tumor necrosis factor messenger RNA/protein was also reduced in C6(-) OLTs in comparison with C6(+) OLTs. Western blot-assisted expression of proapoptotic caspase-3 was decreased in C6(-) OLTs versus C6(+) OLTs (P = 0.006), whereas antiapoptotic Bcl-2/Bag-1 was enhanced in C6(-) OLTs compared with C6(+) OLTs (P = 0.001). Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling staining of apoptotic cells was enhanced (P < 0.05) in C6(+) OLTs compared with C6(-) OLTs. Thus, the terminal products of the complement system are essential in the mechanism of hepatic IRI. This is the first report using a clinically relevant liver cold ischemia model to show that local MAC inhibition attenuates IRI cascade in OLT recipients.

Indexed as

Liver TransplantationAnimalsApoptosisCold IschemiaComplement C6Complement Membrane Attack ComplexCytokinesFemaleLiverLiver DiseasesMacrophagesNeutrophil InfiltrationRatsReperfusion InjuryComplement C6Complement Membrane Attack ComplexCytokines

Identifiers

PMID18668646
PMCPMC2975482
OpenAlexW2046015534

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.